LaborQ, University of Bucharest, 030018 Bucharest, Romania.
Department of Analytical Chemistry and Physical Chemistry, University of Bucharest, 030018 Bucharest, Romania.
Biosensors (Basel). 2023 Oct 25;13(11):954. doi: 10.3390/bios13110954.
A highly sensitive electrochemical biosensor for ethanol based on a screen-printed electrode modified with gold nanoparticles-electrochemically reduced graphene oxide-poly (allylamine hydrochloride) nanocomposite (AuNPs-ERGO-PAH) is reported in this work. Ethanol was oxidized in the presence of the oxidized form of the nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by alcohol dehydrogenase (ADH) immobilized in sol-gel. The AuNPs-ERGO-PAH nanocomposite was used as a transducer for the electrocatalytic oxidation of the reduced form the nicotinamide adenine dinucleotide (NADH) produced in the enzyme reaction. Under the optimal conditions, the ethanol biosensor exhibits a wide dynamic range from 0.05 to 5 mM with a low detection limit of 10 µM (S/N = 3) and a high sensitivity of 44.6 ± 0.07 µA/mM·cm for the linear range between 0.05 and 0.2 mM. The biosensor response was stable for up to 6 weeks. Furthermore, the developed biosensor has been used to detect ethanol in alcoholic beverages with good results, suggesting its potential application in various fields, including fermentation processes and food quality control.
本文报道了一种基于金纳米粒子-电化学还原氧化石墨烯-聚烯丙基胺盐酸盐纳米复合材料（AuNPs-ERGO-PAH）修饰的丝网印刷电极的高灵敏度乙醇电化学生物传感器。在乙醇脱氢酶（ADH）固定化的溶胶-凝胶中，在烟酰胺腺嘌呤二核苷酸（NAD）的氧化形式存在下，乙醇被氧化。AuNPs-ERGO-PAH 纳米复合材料被用作电催化氧化酶反应中产生的还原型烟酰胺腺嘌呤二核苷酸（NADH）的传感器。在最佳条件下，乙醇生物传感器在 0.05 至 5 mM 的宽动态范围内具有低检测限 10 µM（S/N = 3）和高灵敏度 44.6 ± 0.07 µA/mM·cm，线性范围在 0.05 至 0.2 mM 之间。生物传感器的响应在长达 6 周的时间内保持稳定。此外，该开发的生物传感器已用于检测酒精饮料中的乙醇，结果良好，表明其在发酵过程和食品质量控制等各个领域具有潜在的应用。
