Zhao Jianyun, Chen Hu, Li Gaiping, Jumaturti Maimaiti Aisha, Yao Xiaomin, Hu Ying
Key Laboratory of National Forestry and Grassland Administration on Cultivation of Fast-Growing Timber in Central South China, College of Forestry, Guangxi University, Nanning 530004, China.
Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning 530004, China.
Curr Issues Mol Biol. 2023 Nov 16;45(11):9234-9251. doi: 10.3390/cimb45110578.
Magnoliaceae, a family of perennial woody plants, contains several endangered species whose taxonomic status remains ambiguous. The study of chloroplast genome information can help in the protection of Magnoliaceae plants and confirmation of their phylogenetic relationships. In this study, the chloroplast genomes were sequenced, assembled, and annotated in and three Michelia species (, , and ). Comparative analyses of genomic characteristics, repetitive sequences, and sequence differences were performed among the four Magnoliaceae plants, and phylogenetic relationships were constructed with twenty different magnolia species. The length of the chloroplast genomes varied among the four studied species ranging from 159,838 bp () to 160,127 bp (). Four distinct hotspot regions were identified based on nucleotide polymorphism analysis. They were , , , and . These gene fragments may be developed and utilized as new molecular marker primers. By using and as outgroups reference, a phylogenetic tree of the four Magnoliaceae species and eighteen other Magnoliaceae species was constructed with the method of Shared Coding Sequences (). Results showed that the endangered species, is relatively genetically distinct from the other three species, indicating the different phylogenetic processes among Magnoliaceae plants. Therefore, further genetic information is required to determine the relationships within Magnoliaceae. Overall, complete chloroplast genome sequences for four Magnoliaceae species reported in this paper have shed more light on phylogenetic relationships within the botanical group.
木兰科是一类多年生木本植物，其中包含几种濒危物种，其分类地位仍不明确。叶绿体基因组信息的研究有助于保护木兰科植物并确定它们的系统发育关系。在本研究中，对木兰属的 和三种含笑属植物（ 、 和 ）的叶绿体基因组进行了测序、组装和注释。对这四种木兰科植物的基因组特征、重复序列和序列差异进行了比较分析，并与二十种不同的木兰属物种构建了系统发育关系。在所研究的四个物种中，叶绿体基因组的长度各不相同，范围从159,838 bp（ ）到160,127 bp（ ）。基于核苷酸多态性分析确定了四个不同的热点区域。它们是 、 、 和 。这些基因片段可被开发并用作新的分子标记引物。以 和 作为外类群参考，采用共享编码序列（ ）方法构建了四种木兰科物种和其他十八种木兰科物种的系统发育树。结果表明，濒危物种 在遗传上与其他三个物种相对不同，这表明木兰科植物之间的系统发育过程不同。因此，需要更多的遗传信息来确定木兰科内部的关系。总体而言，本文报道的四种木兰科物种的完整叶绿体基因组序列为该植物类群的系统发育关系提供了更多线索。
