Lyons G, Bilgeri Y R, Zanzinger A, Berzin M, Mendelsohn D
Andrologia. 1986 Sep-Oct;18(5):455-60. doi: 10.1111/j.1439-0272.1986.tb01808.x.
This study attempts to provide guidelines for the extraction and estimation of ATP from spermatozoa. The conditions of extraction of ATP from spermatozoa using trichloroacetic acid were optimized. The efficiency of this extraction procedure was compared to that using boiling Tris buffer. Three commercially available kits which use the firefly luciferase assay for ATP and two luminometers were evaluated for the measurement of the spermatozoa extracts. Elevated levels of Mg2+ and/or Ca2+ did not interfere with the extraction of ATP by trichloroacetic acid or with the subsequent estimation of the nucleotide.
本研究旨在为从精子中提取和估算三磷酸腺苷（ATP）提供指导方针。优化了使用三氯乙酸从精子中提取ATP的条件。将该提取程序的效率与使用煮沸的Tris缓冲液的提取程序的效率进行了比较。对三种使用萤火虫荧光素酶法测定ATP的市售试剂盒和两种发光计进行了评估，以测量精子提取物。镁离子（Mg2+）和/或钙离子（Ca2+）水平升高不会干扰三氯乙酸对ATP的提取或随后对该核苷酸的估算。
