解偶联蛋白 3 促进 C2C12 细胞中 IIb 型肌管的成肌分化。
Uncoupling Protein 3 Promotes the Myogenic Differentiation of Type IIb Myotubes in C2C12 Cells.
机构信息
College of Animal Science, Shanxi Agricultural University, 1 Mingxian Nanlu, Jinzhong 030801, China.
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
出版信息
Genes (Basel). 2023 Nov 7;14(11):2049. doi: 10.3390/genes14112049.
Uncoupling protein 3 (Ucp3) is an important transporter within mitochondria and is mainly expressed in skeletal muscle, brown adipose tissue and the myocardium. However, the effects of Ucp3 on myogenic differentiation are still unclear. This study evaluated the effects of Ucp3 on myogenic differentiation, myofiber type and energy metabolism in C2C12 cells. Gain- and loss-of-function studies revealed that 3 could increase the number of myotubes and promote the myogenic differentiation of C2C12 cells. Furthermore, Ucp3 promoted the expression of the type IIb myofiber marker gene myosin heavy chain 4 (4) and decreased the expression of the type I myofiber marker gene myosin heavy chain 7 (7). In addition, energy metabolism related to the expression of PPARG coactivator 1 alpha (1-), ATP synthase, H transportation, mitochondrial F1 complex, alpha subunit 1 (51), lactate dehydrogenase A () and lactate dehydrogenase B () increased with 3 overexpression. Ucp3 could promote the myogenic differentiation of type IIb myotubes and accelerate energy metabolism in C2C12 cells. This study can provide the theoretical basis for understanding the role of Ucp3 in energy metabolism.
解偶联蛋白 3（Ucp3）是线粒体中的一种重要转运蛋白，主要表达于骨骼肌、棕色脂肪组织和心肌。然而，Ucp3 对成肌分化的影响尚不清楚。本研究评估了 Ucp3 对 C2C12 细胞成肌分化、肌纤维类型和能量代谢的影响。获得和丧失功能研究表明，Ucp3 可以增加肌管的数量并促进 C2C12 细胞的成肌分化。此外，Ucp3 促进了 IIb 型肌纤维标记基因肌球蛋白重链 4（4）的表达，并降低了 I 型肌纤维标记基因肌球蛋白重链 7（7）的表达。此外，与表达相关的能量代谢 PPARG 共激活因子 1α（1-α）、ATP 合酶、H 运输、线粒体 F1 复合物、α亚基 1（51）、乳酸脱氢酶 A（）和乳酸脱氢酶 B（）的表达随着 Ucp3 的过表达而增加。Ucp3 可以促进 C2C12 细胞中 IIb 型肌管的成肌分化并加速能量代谢。本研究可为理解 Ucp3 在能量代谢中的作用提供理论基础。
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