MethylRAD Sequencing Technology Reveals DNA Methylation Characteristics of of Different Ages.

The industry has expanded significantly, but no research has focused on determining the age of during farming. Correctly estimating the age of can provide a decision-making basis for the breeding process and data for the protection of aquatic germplasm resources. DNA methylation levels in the body wall of Apostichopus japonicus at 4 months, 1 year, 2 years, and 3 years old were determined using MethylRAD-Seq, and differentially methylated genes were screened. A total of 441 and 966 differentially methylated genes were detected at the CCGG and CCWGG sites, respectively. Aspartate aminotransferase, succinate semialdehyde dehydrogenase, isocitrate dehydrogenase, the histone , heat shock protein , aminopeptidase N, cell division cycle , Ras GTPase activating protein (), slit guidance ligand , integrin-linked kinase , mechanistic target of rapamycin kinase , protein kinase A , and autophagy-related 3 genes may play key roles in the growth and aging process of . This study provides valuable information regarding age-related genes for future research, and these candidate genes can be used to create an "epigenetic clock".