Beckett Linda M, Malacco Victor M R, Hilger Susan, Casey Theresa M, Donkin Shawn S
Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.
College of Agriculture, Oregon State University, Corvallis, OR 97331, USA.
Animals (Basel). 2023 Nov 19;13(22):3572. doi: 10.3390/ani13223572.
Palmitic (C16:0), α-linolenic acid (C18:3n-3 ), and propionate regulate bovine pyruvate carboxylase () and phosphoenolpyruvate carboxykinase () expression in vitro. The objective of this experiment was to determine the impact of C16:0, C18:3n-3 , propionate, and acetate postruminal infusions on hepatic and expression. We hypothesized that circulating fatty acids alter hepatic and in lactating dairy cows. Acetate, propionate, palm oil, and flaxseed oil were supplied postruminally to lactating cows ( = 4) using two 4 × 4 Latin square studies. For Experiment 1, cows were infused on an hourly basis with either a bolus of propionate, acetate, or the combination of propionate and palm oil, or acetate and palm oil, and Experiment 2 was similar, but flaxseed oil replaced palm oil. Flaxseed infusions increased plasma concentration and the molar percent of C18:3n-3 and decreased C16:0 but did not affect or expression. Palm infusions did not affect blood metabolites or the hepatic expression of or . The lack of responses to short-chain fatty acid infusions and changes in circulating long-chain fatty acids in mature cattle are not suitable models to study the effects of α-linolenic acid and propionate on bovine and expression previously observed in vitro.
棕榈酸（C16:0）、α-亚麻酸（C18:3n-3）和丙酸在体外调节牛丙酮酸羧化酶（）和磷酸烯醇式丙酮酸羧激酶（）的表达。本实验的目的是确定瘤胃后注入C16:0、C18:3n-3、丙酸和乙酸对肝脏和表达的影响。我们假设循环脂肪酸会改变泌乳奶牛肝脏中的和。使用两项4×4拉丁方研究，将乙酸、丙酸、棕榈油和亚麻籽油经瘤胃后供给泌乳奶牛（n = 4）。在实验1中，每小时给奶牛注入一次丙酸、乙酸或丙酸与棕榈油的组合，或乙酸与棕榈油的组合，实验2与之类似，但用亚麻籽油替代了棕榈油。注入亚麻籽会增加血浆中C18:3n-3的浓度和摩尔百分比，并降低C16:0，但不影响和的表达。注入棕榈油不影响血液代谢物或肝脏中或的表达。成熟牛对短链脂肪酸注入缺乏反应以及循环长链脂肪酸的变化，并非研究先前在体外观察到的α-亚麻酸和丙酸对牛和表达影响的合适模型。
