College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China.
Potato Research and Development Center, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2023 Nov 8;24(22):16087. doi: 10.3390/ijms242216087.
Potato is an important food crop worldwide. Brassinosteroids (BRs) are widely involved in plant growth and development, and BIN2 (brassinosteroid insensitive 2) is the negative regulator of their signal transduction. However, the function of BIN2 in the formation of potato tubers remains unclear. In this study, transgenic methods were used to regulate the expression level of in plants, and tuber related phenotypes were analyzed. The overexpression of significantly increased the number of potatoes formed per plant and the weight of potatoes in transgenic plants. In order to further explore the effect of StBIN2 on the formation of potato tubers, this study analyzed BRs, ABA hormone signal transduction, sucrose starch synthase activity, the expression levels of related genes, and interacting proteins. The results show that the overexpression of enhanced the downstream transmission of ABA signals. At the same time, the enzyme activity of the sugar transporter and the expression of synthetic genes were increased in potato plants overexpressing , which also demonstrated the upregulation of sucrose and the expression of the starch synthesis gene. Apparently, StBIN2 affected the conversion and utilization of key substances such as glucose, sucrose, and starch in the process of potato formation so as to provide a material basis and energy preparation for forming potatoes. In addition, StBIN2 also promoted the expression of the tuber formation factors StSP6A and StS6K. Altogether, this investigation enriches the study on the mechanism through which StBIN2 regulates potato tuber formation and provides a theoretical basis for achieving a high and stable yield of potato.
马铃薯是全球重要的粮食作物之一。油菜素内酯（BRs）广泛参与植物的生长和发育，而 BIN2（油菜素内酯不敏感 2）是其信号转导的负调控因子。然而，BIN2 在马铃薯块茎形成中的功能尚不清楚。本研究采用转基因方法调控 在植物中的表达水平，并分析块茎相关表型。过表达 显著增加了转基因植株每株形成的马铃薯数量和马铃薯的重量。为了进一步探讨 StBIN2 对马铃薯块茎形成的影响，本研究分析了油菜素内酯、ABA 激素信号转导、蔗糖淀粉合酶活性、相关基因和互作蛋白的表达水平。结果表明，过表达 增强了 ABA 信号的下游传递。同时，过表达 的马铃薯植株中转糖体的酶活性和合成基因的表达增加，这也表明蔗糖的上调和淀粉合成基因的表达。显然，StBIN2 影响了葡萄糖、蔗糖和淀粉等关键物质在马铃薯形成过程中的转化和利用，为形成马铃薯提供了物质基础和能量准备。此外，StBIN2 还促进了块茎形成因子 StSP6A 和 StS6K 的表达。总之，这项研究丰富了 StBIN2 调控马铃薯块茎形成的机制研究，为实现马铃薯的高产和稳产提供了理论依据。
