Division of Nephrology, Department of Internal Medicine, Kaohsiung Municipal Feng-Shan Hospital (Under Management of Chang Gung Medical Foundation), Kaohsiung 83062, Taiwan.
Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan.
Int J Mol Sci. 2023 Nov 17;24(22):16455. doi: 10.3390/ijms242216455.
The kidney plays a crucial role in glucose homeostasis by regulating glucose transport. We aimed to investigate the impact of alterations in glucose transport on glucose metabolism during ageing. Adult male Sprague Dawley rats were divided into five groups: 3-month, 6-month, and 12-month control groups, and 6- and 12-month groups receiving the hydrogen sulfide donor molecule GYY4137. The study found that, as age increased, daily urinary uric acid and protein levels increased in the 12-month group. Blood sugar level and HOMA-IR index increased in the 12-month group, and were partially improved by GYY4137. The kidney tissue showed mild glomerulosclerosis in the 12-month group, which was diminished by GYY4137. Gene expression analysis showed decreased sirtuin and increased p21 expression in the aging groups. Increased SGLT1 and SGLT2 expression was observed in the 12-month group, which was reversed by GYY4137. Both GLUT1 and GLUT2 expression was increased in the 6- and 12-month groups, and reversed by GYY4137 in the 12-month group. The study concluded that aging was associated with increased blood sugar levels and the HOMA-IR index, and the abundance of renal glucose transporters increased as aging progressed. GYY4137 effectively reversed aging-related alterations in glucose homeostasis and renal epithelial transporters.
肾脏通过调节葡萄糖转运来在葡萄糖稳态中发挥关键作用。我们旨在研究葡萄糖转运的改变对衰老过程中葡萄糖代谢的影响。成年雄性 Sprague Dawley 大鼠被分为五组：3 个月、6 个月和 12 个月的对照组，以及接受硫化氢供体分子 GYY4137 的 6 个月和 12 个月组。研究发现，随着年龄的增长，12 个月组的大鼠每日尿尿酸和蛋白质水平增加。12 个月组的血糖水平和 HOMA-IR 指数升高，而 GYY4137 部分改善了这些情况。肾脏组织在 12 个月组中显示出轻度的肾小球硬化，而 GYY4137 减轻了这种情况。基因表达分析显示，衰老组中的沉默调节蛋白和 p21 表达增加。在 12 个月组中观察到 SGLT1 和 SGLT2 表达增加，而 GYY4137 逆转了这种情况。6-和 12 个月组的 GLUT1 和 GLUT2 表达均增加，而 GYY4137 在 12 个月组中逆转了这种情况。研究结论是，衰老与血糖水平和 HOMA-IR 指数升高有关，随着衰老的进展，肾脏葡萄糖转运体的丰度增加。GYY4137 有效地逆转了与衰老相关的葡萄糖稳态和肾脏上皮转运体的改变。
