Goldkamp Anna K, Lahuis Ciarra H, Hagen Darren E, Taxis Tasia M
Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK 74074, USA.
Department of Animal Science, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI 48824, USA.
Pathogens. 2023 Nov 3;12(11):1312. doi: 10.3390/pathogens12111312.
Small non-coding RNAs, such as microRNAs (miRNA) and tRNA-derived fragments (tRF), are known to be involved in post-transcriptional gene regulation. Research has provided evidence that small RNAs may influence immune development in calves. Bovine leukosis is a disease in cattle caused by Bovine Leukemia Virus (BLV) that leads to increased susceptibility to opportunistic pathogens. No research has addressed the potential influence that a maternal BLV infection may have on gene regulation through the differential expression of miRNAs or tRFs in progeny. Blood samples from 14-day old Holstein calves born to BLV-infected dams were collected. Antibodies for BLV were assessed using ELISA and levels of BLV provirus were assessed using qPCR. Total RNA was extracted from whole blood samples for small RNA sequencing. Five miRNAs (bta-miR-1, bta-miR-206, bta-miR-133a, bta-miR-133b, and bta-miR-2450d) and five tRFs (tRF-36-8JZ8RN58X2NF79E, tRF-20-0PF05B2I, tRF-27-W4R951KHZKK, tRF-22-S3M8309NF, and tRF-26-M87SFR2W9J0) were dysregulated in calves born to BLV-infected dams. The miRNAs appear to be involved in the gene regulation of immunological responses and muscle development. The tRF subtypes and parental tRNA profiles in calves born to infected dams appear to be consistent with previous publications in adult cattle with BLV infection. These findings offer insight into how maternal BLV infection status may impact the development of offspring.
已知微小RNA（miRNA）和tRNA衍生片段（tRF）等小非编码RNA参与转录后基因调控。研究已提供证据表明小RNA可能影响犊牛的免疫发育。牛白血病是一种由牛白血病病毒（BLV）引起的牛病，会导致对机会性病原体的易感性增加。尚无研究探讨母体BLV感染可能通过子代中miRNA或tRF的差异表达对基因调控产生的潜在影响。收集了感染BLV的母牛所生14日龄荷斯坦犊牛的血样。使用酶联免疫吸附测定（ELISA）评估BLV抗体，并使用定量聚合酶链反应（qPCR）评估BLV前病毒水平。从全血样本中提取总RNA用于小RNA测序。在感染BLV的母牛所生的犊牛中，有5种miRNA（bta-miR-1、bta-miR-206、bta-miR-133a、bta-miR-133b和bta-miR-2450d）和5种tRF（tRF-36-8JZ8RN58X2NF79E、tRF-20-0PF05B2I、tRF-27-W4R951KHZKK、tRF-22-S3M8309NF和tRF-26-M87SFR2W9J0）表达失调。这些miRNA似乎参与免疫反应和肌肉发育的基因调控。感染母牛所生犊牛中的tRF亚型和亲本tRNA谱似乎与先前关于感染BLV的成年牛的出版物一致。这些发现为母体BLV感染状态如何影响后代发育提供了见解。
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