Zhao Xueyan, Wang Li, Zhou Yafu, Wang Qing, Wang Fangyuan, Li Yan
Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources, Xi'an Botanical Garden of Shaanxi Province (Institute of Botany of Shaanxi Province), Xi'an 710061, China.
Life (Basel). 2023 Nov 14;13(11):2207. doi: 10.3390/life13112207.
is a medicinal herb in China that has been widely used to treat various kinds of pain. The tuber is the main organ of used for medicinal purposes, but changes in related genes during the development of the tuber have rarely been reported. To identify the differentially expressed genes during tuber development, full-length transcriptomic sequencing was performed using single-molecule real-time technology, and tubers at three development stages were selected for comparative transcriptome analysis. A total of 90,496 full-length non-chimeric transcripts were obtained, and 19,341 transcripts were annotated in at least one public database. A total of 9221 differentially expressed genes were identified during the swelling process of tuber. A Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis revealed that differentially expressed genes associated with a "starch and sucrose metabolism pathway", "phenylpropanoid biosynthesis pathway", "isoquinoline alkaloid biosynthesis pathway", "zeatin biosynthesis pathway", and "brassinosteroid biosynthesis pathway" were predominantly enriched. In addition, the genes involved in cell wall metabolism were potentially associated with tuber swelling. These processes regulated and were involved in tuber development. The results provide a foundation for further research on tuber formation in medicinal plants.
在中国，它是一种药用植物，已被广泛用于治疗各种疼痛。块茎是其用于药用的主要器官，但块茎发育过程中相关基因的变化鲜有报道。为了鉴定块茎发育过程中的差异表达基因，利用单分子实时技术进行了全长转录组测序，并选择了三个发育阶段的块茎进行比较转录组分析。共获得90496条全长非嵌合转录本，其中19341条转录本在至少一个公共数据库中得到注释。在其块茎膨大过程中，共鉴定出9221个差异表达基因。京都基因与基因组百科全书（KEGG）通路富集分析表明，与“淀粉和蔗糖代谢途径”“苯丙烷生物合成途径”“异喹啉生物碱生物合成途径”“玉米素生物合成途径”和“油菜素内酯生物合成途径”相关的差异表达基因显著富集。此外，参与细胞壁代谢的基因可能与块茎膨大有关。这些过程调节并参与了其块茎的发育。研究结果为进一步研究药用植物块茎形成提供了基础。
