Hata Noriko, Takahashi Seira, Osada Sachiko, Katagiri Sakura, Naruse Mayumi, Igarashi Akane, Sazawa Kazuto, Taguchi Shigeru, Kuramitz Hideki
Major of Earth, Life and Environmental Science, Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
Molecules. 2023 Nov 10;28(22):7525. doi: 10.3390/molecules28227525.
The design of a simple approach enabling the detection of bisphenol A (BPA) in water samples without the need for large amounts of solvents is of utmost importance. This paper reports a simple method for the separation, concentration, and quantification of BPA in water samples using high-performance liquid chromatography with fluorescence detection (HPLC-FLD) after its microextraction into an in situ formed organic ion-associate (IA) liquid phase (LP). Novel IA phase components without conjugated double bonds, such as benzene rings, were investigated. Ethylhexyloxypropylamine hydrochloride and sodium dodecyl sulfate solutions were added to the water samples to form IAs. The aqueous phase and ion-associate liquid phase (IALP) were separated by centrifugation. The aqueous phase was removed, and the liquid phase was recovered and measured using HPLC-FLD or HPLC-electrochemical detection (ECD). The concentrated phase (IALP) had a relatively low viscosity and could be injected directly into the chromatograph without dissolving it in organic solvents. The detection limits for BPA by HPLC-FLD and HPLC-ECD were 0.009 and 0.3 µg L, respectively.
设计一种简单的方法，无需大量溶剂就能检测水样中的双酚A（BPA）至关重要。本文报道了一种简单的方法，用于水样中双酚A的分离、浓缩和定量。该方法是先将双酚A微萃取到原位形成的有机离子缔合物（IA）液相（LP）中，然后采用带荧光检测的高效液相色谱法（HPLC-FLD）进行分析。研究了不含共轭双键（如苯环）的新型IA相成分。向水样中加入盐酸乙基己氧基丙胺和十二烷基硫酸钠溶液以形成离子缔合物。通过离心分离水相和离子缔合液相（IALP）。去除水相，回收液相并使用HPLC-FLD或HPLC-电化学检测（ECD）进行测量。浓缩相（IALP）具有相对较低的粘度，无需溶解在有机溶剂中即可直接注入色谱仪。HPLC-FLD和HPLC-ECD对双酚A的检测限分别为0.009和0.3 μg/L。
