基于中国东部汉族人群的 DNA 甲基化年龄估算模型的跨平台应用。
Cross-Platform Application of DNA Methylation Age Estimation Model in Eastern Chinese Han Population.
机构信息
Department of Forensic Medicine, School of Basic Medicine and Biological Sciences, Suzhou Medical College of Soochow University, Suzhou 215123, China.
Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
出版信息
Fa Yi Xue Za Zhi. 2023 Oct 25;39(5):441-446. doi: 10.12116/j.issn.1004-5619.2022.520405.
OBJECTIVES
To evaluate the forensic application value of an age estimation model based on DNA methylation in eastern Chinese Han population, and to provide a theoretical basis for exploring age estimation models suitable for different detection platforms.
METHODS
According to the 6 age-related methylation sites in the published blood DNA methylation age estimation models of Chinese Han population, the DNA methylation level of 48 samples was detected by pyrosequencing and next-generation sequencing (NGS). After submitting DNA methylation levels to the age estimation model, the DNA methylation ages were predicted and compared with their real ages.
RESULTS
The 6 DNA methylation sites in both detection techniques were age-related, with an of 0.85 and a median absolute deviation (MAD) of 4.81 years when using pyrosequencing；with an of 0.84 and MAD of 4.41 years when using NGS.
CONCLUSIONS
The blood DNA methylation age estimation model can be used under pyrosequencing and multi-purpose regional methylation enrichment sequencing technology based on NGS and it can accurately estimate the age.
目的
评估基于中国人东汉族人群 DNA 甲基化的法医年龄估测模型的应用价值，为探索适用于不同检测平台的年龄估测模型提供理论依据。
方法
根据已发表的中国人汉族人群血液 DNA 甲基化年龄估测模型中的 6 个年龄相关甲基化位点，采用焦磷酸测序和下一代测序（NGS）检测 48 例样本的 DNA 甲基化水平。将 DNA 甲基化水平提交至年龄估测模型后，预测 DNA 甲基化年龄并与实际年龄进行比较。
结果
两种检测技术中的 6 个 DNA 甲基化位点均与年龄相关，焦磷酸测序的相关系数（）为 0.85，中值绝对偏差（MAD）为 4.81 岁；NGS 多用途区域甲基化富集测序技术的相关系数（）为 0.84，MAD 为 4.41 岁。
结论
该血液 DNA 甲基化年龄估测模型可应用于焦磷酸测序和基于 NGS 的多用途区域甲基化富集测序技术，可准确估算年龄。
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