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植物寄生线虫中沃尔巴克氏体的检测与分析及沃尔巴克氏体进化的研究进展

Detection and Analysis of Wolbachia in Plant-Parasitic Nematodes and Insights into Wolbachia Evolution.

机构信息

Department of Biological Sciences, Texas Tech University, Lubbock, TX, USA.

出版信息

Methods Mol Biol. 2024;2739:115-134. doi: 10.1007/978-1-0716-3553-7_7.

Abstract

Since the discovery of Wolbachia in plant-parasitic nematodes (PPNs), there has been increased interest in this earliest branching clade that may hold important clues to early transitions in Wolbachia function in the Ecdysozoa. However, due to the specialized skills and equipment of nematology and the difficulty in culturing most PPNs, these PPN-type Wolbachia remain undersampled and poorly understood. To date, there are few established laboratory methods for working with PPN-type Wolbachia strains, and most research has relied on chance discovery and comparative genomics. Here, we address this challenge by providing detailed methods to assist researchers with more efficiently collecting PPNs and screen these communities, populations, or single nematodes with a newly developed PPN-type Wolbachia-specific PCR assay. We provide an overview of the typical yields and outcomes of these methods, to facilitate further targeted cultivation or experimental methods, and finally we provide a short introduction to some of the specific challenges and solutions in following through with comparative or population genomics on PPN-type Wolbachia strains.

摘要

自植物寄生线虫(PPN)中发现 Wolbachia 以来,人们对这个最早分支的类群越来越感兴趣,因为它可能为节肢动物中 Wolbachia 功能的早期转变提供了重要线索。然而,由于线虫学的专业技能和设备以及大多数 PPN 的培养难度,这些 PPN 型 Wolbachia 的样本仍然较少,人们对其了解也较少。迄今为止,很少有针对 PPN 型 Wolbachia 菌株的成熟实验室方法,大多数研究依赖于偶然发现和比较基因组学。在这里,我们通过提供详细的方法来解决这一挑战,以帮助研究人员更有效地收集 PPN,并使用新开发的 PPN 型 Wolbachia 特异性 PCR 检测方法对这些群落、种群或单个线虫进行筛选。我们概述了这些方法的典型产量和结果,以促进进一步的靶向培养或实验方法,最后我们还简要介绍了在对 PPN 型 Wolbachia 菌株进行比较或种群基因组学研究时可能遇到的具体挑战和解决方案。

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