淫羊藿素和间充质干细胞衍生的细胞外囊泡联合在藻酸盐-透明质酸水凝胶中增强软骨再生。
Enhanced cartilage regeneration by icariin and mesenchymal stem cell-derived extracellular vesicles combined in alginate-hyaluronic acid hydrogel.
机构信息
School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, PR China.
Department of Ultrasound, Institute of Ultrasound in Musculoskeletal Sports Medicine, Guangdong Second Provincial General Hospital, 510317 Guangzhou, PR China.
出版信息
Nanomedicine. 2024 Jan;55:102723. doi: 10.1016/j.nano.2023.102723. Epub 2023 Nov 23.
OBJECTIVE
Osteoarthritis (OA) is characterized by progressive cartilage degeneration and absence of curative therapies. Therefore, more efficient therapies are compellingly needed. Both mesenchymal stem cells (MSCs)-derived extracellular vesicles (EVs) and Icariin (ICA) are promising for repair of cartilage defect. This study proposes that ICA may be combined to potentiate the cartilage repair capacity of MSC-EVs.
MATERIALS AND METHODS
MSC-EVs were isolated from sodium alginate (SA) and hyaluronic acid (HA) composite hydrogel (SA-HA) cell spheroid culture. EVs and ICA were combined in SA-HA hydrogel to test therapeutic efficacy on cartilage defect in vivo.
RESULTS
EVs and ICA were synergistic for promoting both proliferation and migration of MSCs and inflammatory chondrocytes. The combination therapy led to strikingly enhanced repair on cartilage defect in rats, with mechanisms involved in the concomitant modulation of both cartilage degradation and synthesis makers.
CONCLUSION
The MSC-EVs-ICA/SA-HA hydrogel potentially constitutes a novel therapy for cartilage defect in OA.
目的
骨关节炎（OA）的特征是进行性软骨退化，且缺乏有效的治疗方法。因此，迫切需要更有效的治疗方法。间充质干细胞（MSCs）衍生的细胞外囊泡（EVs）和淫羊藿苷（ICA）在修复软骨缺损方面都很有前景。本研究提出，ICA 可能与 MSC-EVs 联合使用以增强其软骨修复能力。
材料与方法
从海藻酸钠（SA）和透明质酸（HA）复合水凝胶（SA-HA）细胞球培养中分离 MSC-EVs。将 EVs 和 ICA 结合到 SA-HA 水凝胶中，以测试体内软骨缺损的治疗效果。
结果
EVs 和 ICA 协同促进了 MSCs 和炎症软骨细胞的增殖和迁移。联合治疗对大鼠的软骨缺损有显著的修复作用，其机制涉及对软骨降解和合成标志物的同时调节。
结论
MSC-EVs-ICA/SA-HA 水凝胶可能为 OA 中的软骨缺损提供一种新的治疗方法。
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