College of Life Science, Yantai University, Yantai, Shandong, 264005, China.
Qingdao International Travel Healthcare Center, Qingdao, Shandong, 266071, China.
Sci Data. 2023 Nov 25;10(1):829. doi: 10.1038/s41597-023-02655-5.
Przevalski's partridge (Alectoris magna) is one of the birds in the genus Alectoris endemic to China. The distribution of A. magna was narrow, and it was only found in parts of the Qinghai, Gansu, and Ningxia provinces. A. magna was considered a monotypic species until it was distinguished into two subspecies. However, external morphological characteristics, rather than genetic differences or evolutionary relationships, are now commonly used as evidence of subspecies differentiation. In this study, a chromosome-level reference genome of A. magna has been constructed by combining Illumina, PacBio and Hi-C sequencing data. The 1135.01 Mb A. magna genome was ultimately assembled. The genome showed 96.9% completeness (BUSCO), with a contig N50 length of 23.34 Mb. The contigs were clustered and oriented on 20 chromosomes, covering approximately 99.96% of the genome assembly. Additionally, altogether 19,103 protein-coding genes were predicted, of which 95.10% were functionally annotated. This high-quality genome assembly could serve as a valuable genomic resource for future research on the functional genomics, genetic protection, and interspecific hybridization of A. magna.
普氏原羚(Alectoris magna)是中国特有的原羚属鸟类之一。普氏原羚的分布范围狭窄,仅分布于青海、甘肃和宁夏的部分地区。普氏原羚曾被认为是单型种,直到后来被分为两个亚种。然而,亚种分化的证据通常是外部形态特征,而不是遗传差异或进化关系。在这项研究中,我们结合 Illumina、PacBio 和 Hi-C 测序数据构建了普氏原羚的染色体水平参考基因组。最终组装得到了 1135.01 Mb 的普氏原羚基因组,其完整性达到 96.9%(BUSCO),contig N50 长度为 23.34 Mb。contigs 被聚类并定向到 20 条染色体上,覆盖了基因组组装的大约 99.96%。此外,总共预测到了 19103 个蛋白质编码基因,其中 95.10%具有功能注释。这个高质量的基因组组装可以作为未来研究普氏原羚功能基因组学、遗传保护和种间杂交的宝贵基因组资源。