Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, Hainan, China.
University of Chinese Academy of Sciences, Beijing, China.
Sci Data. 2023 Aug 1;10(1):506. doi: 10.1038/s41597-023-02397-4.
Understanding of adaptation and evolution of organisms in the deep sea requires more genomic resources. Zoroaster cf. ophiactis is a sea star in the family Zoroasteridae occurring exclusively in the deep sea. In this study, a chromosome-level genome assembly for Z. cf. ophiactis was generated by combining Nanopore long-read, Illumina short-read, and Hi-C sequencing data. The final assembly was 1,002.0 Mb in length, with a contig N50 of 376 Kb and a scaffold N50 of 40.4 Mb, and included 22 pseudo-chromosomes, covering 92.3% of the assembly. Completeness analysis evaluated with BUSCO revealed that 95.91% of the metazoan conserved genes were complete. Additionally, 39,426 protein-coding genes were annotated for this assembly. This chromosome-level genome assembly represents the first high-quality genome for the deep-sea Asteroidea, and will provide a valuable resource for future studies on evolution and adaptation of deep-sea echinoderms.
理解深海生物的适应和进化需要更多的基因组资源。Zoroaster cf. ophiactis 是海星科 Zoroasteridae 中仅存在于深海的一种海星。在这项研究中，通过结合纳米孔长读长、Illumina 短读长和 Hi-C 测序数据，生成了 Z. cf. ophiactis 的染色体水平基因组组装。最终组装的基因组大小为 1002.0 Mb，contig N50 为 376 Kb，scaffold N50 为 40.4 Mb，包含 22 个假染色体，覆盖了组装的 92.3%。使用 BUSCO 进行完整性分析评估表明，后生动物保守基因的完整性达到 95.91%。此外，还为这个组装注释了 39426 个蛋白质编码基因。这个染色体水平的基因组组装代表了深海海星的第一个高质量基因组，将为未来深海棘皮动物的进化和适应研究提供有价值的资源。
