Laboratory of Environmental Hygiene, Department of Health and Sports Science, Juntendo University, Chiba, Japan.
Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, Tokyo, Japan.
J Vet Med Sci. 2024 Jan 26;86(1):92-95. doi: 10.1292/jvms.23-0373. Epub 2023 Nov 27.
Suncus murinus is gaining prominence as a laboratory animal; however, there is no generally accepted method for microbiological monitoring. This study aimed to apply non-serological microbiological monitoring of laboratory mice for S. murinus and identify the subdominant species obtained by culture methods for microbial assessment. Culture and PCR were used to test S. murinus for the laboratory mice test panels including 10 bacterial species and orthohantaviruses, all of which were negative. The species that grew sub-dominantly in rectal feces were identified as Aeromonas hydrophila, which is pathogenic to mammals. These results indicate that microbiological monitoring should be used to detect pathogens directly from S. murinus, not from sentinel animals, due to the host-specific microbial environment.
沙鼠作为一种实验动物正受到越来越多的关注；然而，目前还没有被普遍认可的微生物监测方法。本研究旨在将实验室小鼠的非血清学微生物监测方法应用于沙鼠，并确定通过培养方法获得的用于微生物评估的亚优势种。通过培养和 PCR 对沙鼠进行了包括 10 种细菌和正呼肠孤病毒在内的实验室小鼠检测面板检测，结果均为阴性。在直肠粪便中生长的亚优势种被鉴定为嗜水气单胞菌，该菌对哺乳动物具有致病性。这些结果表明，由于宿主特异性的微生物环境，微生物监测应该直接从沙鼠中检测病原体，而不是从哨兵动物中检测。
