Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Division of Infertility Clinic, Lee Women's Hospital, Taichung, Taiwan.
Division of Infertility Clinic, Lee Women's Hospital, Taichung, Taiwan.
Taiwan J Obstet Gynecol. 2023 Nov;62(6):845-851. doi: 10.1016/j.tjog.2023.07.035.
The aim of this study was to investigate the correlation between hormone receptor levels and telomere length (TL) in infertile women with and without polycystic ovary syndrome (PCOS).
This prospective cohort study recruited a total of 431 cumulus oocyte complex (COC) from 88 infertile women between July 2012 and June 2014. The participants were divided into three groups: young age (<38 years, n = 42 and 227 COC), advanced age (≥38 years, n = 33 and 107 COC) and PCOS patients (n = 13 and 97 COC). Cumulus cells were collected from individual follicle during oocyte pick-up, and the mRNA levels of hormone receptors and TL were measured using real-time PCR.
The cumulus cells of PCOS patients demonstrated lower mRNA levels of LH receptor (75.57 ± 138.10 vs. 171.07 ± 317.68; p < 0.01) and androgen receptor (1.13 ± 1.52 vs. 4.08 ± 9.57; p < 0.01), as well as a shorter TL (2.39 ± 2.58 vs. 3.96 ± 4.72; p < 0.01) compared to those of the young age group. In the young age group, only androgen receptor mRNA level showed a significant association with TL (rho = 0.148, p = 0.026), while FSH receptor mRNA level was the only factor associated with TL (rho = 0.247, p = 0.015) in PCOS patients. For advanced-aged patients, no significant relationship was observed between hormone receptor mRNA levels and TL. Alternative splicing of androgen receptors was identified in some PCOS patients but not in young age controls.
The findings suggest that the androgen receptor level and function may be altered in the cumulus cells of PCOS patients, leading to a shorter TL in cumulus cells in PCOS patients.
本研究旨在探讨不孕妇女中多囊卵巢综合征（PCOS）患者和非 PCOS 患者的激素受体水平与端粒长度（TL）之间的相关性。
这项前瞻性队列研究共招募了 2012 年 7 月至 2014 年 6 月间 88 名不孕妇女的 431 个卵丘-卵母细胞复合物（COC）。将参与者分为三组：年轻组（<38 岁，n=42 和 227 COC）、高龄组（≥38 岁，n=33 和 107 COC）和 PCOS 患者组（n=13 和 97 COC）。在取卵时从单个卵泡中收集卵丘细胞，并使用实时 PCR 测量激素受体和 TL 的 mRNA 水平。
与年轻组相比，PCOS 患者的卵丘细胞中 LH 受体（75.57±138.10 比 171.07±317.68；p<0.01）和雄激素受体（1.13±1.52 比 4.08±9.57；p<0.01）的 mRNA 水平较低，TL 较短（2.39±2.58 比 3.96±4.72；p<0.01）。在年轻组中，只有雄激素受体 mRNA 水平与 TL 呈显著相关（rho=0.148，p=0.026），而在 PCOS 患者中，只有 FSH 受体 mRNA 水平与 TL 相关（rho=0.247，p=0.015）。对于高龄患者，激素受体 mRNA 水平与 TL 之间未观察到显著关系。在一些 PCOS 患者中鉴定出雄激素受体的选择性剪接，但在年轻对照组中没有。
研究结果表明，PCOS 患者卵丘细胞中的雄激素受体水平和功能可能发生改变，导致 PCOS 患者卵丘细胞的 TL 变短。
