Newby A C
Biochem J. 1986 Aug 1;237(3):845-51. doi: 10.1042/bj2370845.
Steady-state mathematical models are developed according to which adenosine is formed in the cytoplasm of a group of cells, arises in the extracellular space via the symmetric nucleoside transporter and is inactivated in the adenosine forming cells and after rate-limiting transport into other cell-types. Dipyridamole increases the Km and Vmax. of the transporter symmetrically with respect to influx and efflux. Models incorporating differing degrees of compartmentation are used to predict intracellular and extracellular adenosine concentration as a function of dipyridamole concentration and adenosine formation rate. The vasodilator action of dipyridamole is explained since it is predicted to elevate interstitial fluid adenosine concentrations at all rates of adenosine formation provided that washout of the interstitial compartment is restricted.
腺苷在一组细胞的细胞质中形成，通过对称核苷转运体出现在细胞外空间，并在形成腺苷的细胞中以及在限速转运到其他细胞类型后失活。双嘧达莫对称地增加了转运体的Km和Vmax，其流入和流出的情况相同。采用包含不同程度分隔的模型来预测细胞内和细胞外腺苷浓度与双嘧达莫浓度及腺苷形成速率的函数关系。双嘧达莫的血管舒张作用得到了解释，因为据预测，只要间质隔室的清除受到限制，在所有腺苷形成速率下，它都会提高间质液中腺苷的浓度。
