Park T S, Gidday J M
Department of Neurosurgery, Washington University School of Medicine, St. Louis Children's Hospital, Missouri 63110.
J Cereb Blood Flow Metab. 1990 May;10(3):424-7. doi: 10.1038/jcbfm.1990.73.
The effect of dipyridamole, an adenosine transport inhibitor, on cerebral extracellular adenosine concentration remains to be determined. To examine this issue, bilateral brain dialysis samples were obtained from piglet frontal cortex before, during, and after 5 min of cerebral ischemia; 10(-4) M dipyridamole was administered through one dialysis probe. On the control side, dialysate adenosine concentration increased 5.7-fold during ischemia and 15-fold during the first 5 min of reperfusion; it returned to control levels after 15 min of reperfusion. Relative to the control side, dipyridamole caused a twofold increase in basal dialysate adenosine concentration and increased dialysate adenosine concentration at 10 and 15 min of reperfusion, but no increase in dialysate adenosine occurred during and immediately after ischemia. The results indicate that, in the piglet brain, cerebral ischemia markedly elevates intracerebral extracellular adenosine concentration and that dipyridamole increases extracellular adenosine levels.
双嘧达莫（一种腺苷转运抑制剂）对脑细胞外腺苷浓度的影响尚待确定。为研究此问题，在仔猪大脑缺血5分钟前、期间及之后，从其额叶皮质获取双侧脑透析样本；通过一个透析探头给予10(-4)M双嘧达莫。在对照侧，透析液腺苷浓度在缺血期间增加5.7倍，在再灌注的最初5分钟内增加15倍；再灌注15分钟后恢复至对照水平。相对于对照侧，双嘧达莫使基础透析液腺苷浓度增加两倍，并在再灌注10分钟和15分钟时增加透析液腺苷浓度，但在缺血期间及缺血后即刻透析液腺苷浓度未增加。结果表明，在仔猪大脑中，脑缺血显著升高脑内细胞外腺苷浓度，且双嘧达莫可增加细胞外腺苷水平。
