宫颈、胃和支气管黏液糖蛋白的电子显微镜检查

Sheehan J K, Oates K, Carlstedt I

Biochem J. 1986 Oct 1;239(1):147-53. doi: 10.1042/bj2390147.

Mucus glycoproteins (mucins) were obtained from human cervical and pig gastric mucus as well as from chronic-bronchitic sputum after low-shear extraction. The mucus gel was solubilized in guanidinium chloride supplemented with proteinase inhibitors, and the macromolecules were purified by using isopycnic density-gradient centrifugation. The macromolecules were spread in monolayers of benzyldimethylalkyl-ammonium chloride and studied with electron microscopy after staining with uranyl acetate and/or shadowing with platinum/carbon. The mucins appeared as flexible linear threads with lengths varying from approx. 200 nm to about 400 nm. No regularly branched or star-shaped structures were observed. The macromolecular architecture of cervical, respiratory and gastric mucins is thus similar.

通过低剪切力提取，从人宫颈黏液、猪胃黏液以及慢性支气管炎痰液中获取黏液糖蛋白（黏蛋白）。将黏液凝胶溶解于添加了蛋白酶抑制剂的氯化胍中，然后通过等密度梯度离心法对大分子进行纯化。将大分子铺展在苄基二甲基烷基氯化铵单层中，在用醋酸铀染色和/或用铂/碳进行投影后，用电子显微镜进行研究。黏蛋白呈现为长度约从200纳米到约400纳米不等的柔性线性细丝。未观察到规则的分支或星形结构。因此，宫颈、呼吸道和胃黏蛋白的大分子结构相似。