Liu Yufei, Cao Ying, Chang Liye, Shan Conghui, Xu Kun
College of Animal Science and Technology, Northwest A & F University, Yangling 712100, Shaanxi, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Nov 25;39(11):4376-4396. doi: 10.13345/j.cjb.230095.
Nowadays, engineered plays an important role in the biosynthesis of small molecule metabolites and protein products, showing great potential and value in industrial productions. With the development and application of new editing tools such as CRISPR/Cas9, it has become possible to engineer . into a cell factory with high polygenic efficiency. Here, the genetic manipulation techniques and objectives for engineering . are first summarized. Secondly, the applications of engineered . as a cell factory are introduced. Meanwhile, the advantages as well as disadvantages of using engineered . as a cell factory are discussed and future engineering directions are prospected. This review aims to provide a reference for further engineering . cell factory, which is supposed to facilitate its application in bioindustry.
如今，工程化在小分子代谢物和蛋白质产物的生物合成中发挥着重要作用，在工业生产中显示出巨大的潜力和价值。随着CRISPR/Cas9等新型编辑工具的开发和应用，将……工程化为具有高多基因效率的细胞工厂已成为可能。在此，首先总结了……的基因操作技术和目标。其次，介绍了工程化……作为细胞工厂的应用。同时，讨论了使用工程化……作为细胞工厂的优缺点，并展望了未来的工程方向。本综述旨在为进一步构建……细胞工厂提供参考，有望促进其在生物产业中的应用。 （注：原文中“engineered”后缺少具体对象，翻译时用“……”表示此处信息缺失）
