Cheng Feng, Li Huan, Li Kexin, Liu Haiyun, Shen Qi, Xue Yaping, Zheng Yuguo
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Nov 25;39(11):4413-4427. doi: 10.13345/j.cjb.230290.
Adenosine triphosphate (ATP) regeneration systems are essential for efficient biocatalytic phosphoryl transfer reactions. Polyphosphate kinase (PPK) is a versatile enzyme that can transfer phosphate groups among adenosine monophosphate (AMP), adenosine diphosphate (ADP), ATP, and polyphosphate (Poly P). Utilization of PPK is an attractive solution to address the problem of ATP regeneration due to its ability to use a variety of inexpensive and stable Poly P salts as phosphate group donors. This review comprehensively summarizes the structural characteristics and catalytic mechanisms of different types of PPKs, as well as the variations in enzyme activity, catalytic efficiency, stability, and coenzyme preference observed in PPKs from different sources. Moreover, recent advances in PPK-mediated ATP regeneration systems and protein engineering of wild-type PPK are summarized.
三磷酸腺苷（ATP）再生系统对于高效生物催化磷酸基转移反应至关重要。多聚磷酸激酶（PPK）是一种多功能酶，可在一磷酸腺苷（AMP）、二磷酸腺苷（ADP）、ATP和多聚磷酸（Poly P）之间转移磷酸基团。由于PPK能够使用多种廉价且稳定的多聚磷酸（Poly P）盐作为磷酸基团供体，因此利用PPK是解决ATP再生问题的一种有吸引力的方法。本综述全面总结了不同类型PPK的结构特征和催化机制，以及在来自不同来源的PPK中观察到的酶活性、催化效率、稳定性和辅酶偏好的变化。此外，还总结了PPK介导的ATP再生系统和野生型PPK蛋白质工程的最新进展。
