Department of Clinical Biochemistry, School of Medicine, Behbahan Faculty of Medical Sciences, Behbahan, Iran.
Department of Biology, Higher Education Institute, Meymeh, Iran.
Women Health. 2024 Jan 2;64(1):32-40. doi: 10.1080/03630242.2023.2286264. Epub 2023 Dec 28.
Cyclophosphamide is a drug used in chemotherapy. However, it has side effects, including changes in reproductive system functioning. Some herbal compounds can reduce the harmful effects of cyclophosphamide. This study aims to investigate the protective role of crocin against changes caused by Cyclophosphamide in ovarian tissue through changes in the expression of genes involved in the hypothalamic-pituitary-gonadal axis. This experimental study was performed on 24 adult female Wistar rats. Mice were divided into four groups (normal saline, 30 mg/kg cyclophosphamide, 100 mg/kg crocin and 30 mg/kg cyclophosphamide, and 200 mg/kg crocin and 30 mg/kg cyclophosphamide). At the end of the treatment period, the hypothalamus and ovaries were also removed to evaluate genes expression using real-time PCR and histological changes in the ovaries. Data were analyzed by SPSS statistical software. The expression of genes, number of follicles, and follicle diameter significantly decreased in the cyclophosphamide-treated groups compared with the control group. In the crocin and cyclophosphamide-treated groups, drug-induced reproductive complications were mitigated. The current findings indicate that by increasing the expression of genes , , and , crocin could modulate the harmful effects of cyclophosphamide.
环磷酰胺是一种用于化疗的药物。然而，它有副作用，包括生殖系统功能的改变。一些草药化合物可以减少环磷酰胺的有害影响。本研究旨在通过改变参与下丘脑-垂体-性腺轴的基因表达，研究西红花苷对环磷酰胺引起的卵巢组织变化的保护作用。这项实验性研究是在 24 只成年雌性 Wistar 大鼠上进行的。将小鼠分为四组（生理盐水、30mg/kg 环磷酰胺、100mg/kg 西红花苷和 30mg/kg 环磷酰胺、200mg/kg 西红花苷和 30mg/kg 环磷酰胺）。在治疗期末，还取出下丘脑和卵巢，使用实时 PCR 评估基因表达，并观察卵巢的组织学变化。数据用 SPSS 统计软件进行分析。与对照组相比，环磷酰胺处理组的基因表达、卵泡数量和卵泡直径显著降低。在西红花苷和环磷酰胺处理组中，药物引起的生殖并发症得到缓解。目前的研究结果表明，西红花苷通过增加基因的表达，可以调节环磷酰胺的有害作用。
