Dong Qingrong, Fang Ge, Liu Fang, Cai Shuwei, Tao Yujie, Xue Tingyu, Tang Minghua, Zhang Kun, An Ziheng, Du Jiangfeng, Zhang Hui
Department of Medical Imaging, First Hospital of Shanxi Medical University, Taiyuan 030001, China.
State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China.
J Mater Chem B. 2023 Dec 13;11(48):11578-11587. doi: 10.1039/d3tb02065g.
Chronic wound healing remains challenging due to the oxidative microenvironment. Prussian blue (PB) nanoparticles exhibiting multiple antioxidant enzyme-like activities have attracted widespread attention, while their antioxidant efficacy remains unsatisfied. Herein, ultrasmall calcium-enriched Prussian blue nanoparticles (CaPB NPs) are simply constructed with high yields for the wound repair application. Owing to the ultrasmall size and synergistic effect of the generated dual active sites, the CaPB NPs exhibit prominent antioxidase-like activities, protecting cells from oxidative stress-induced damage. In addition to the effect of Ca on regulating keratinocyte and fibroblast growth, it has been demonstrated that the administration of CaPB NPs obviously promoted wound closure as well as collagen deposition and neovascularization in the full-thickness wound defect model in mice. Importantly, the CaPB NP treatment can effectively up-regulate the expression levels of anti-inflammatory cytokines and vascular endothelial growth factors to remodel the wound microenvironment, thereby accelerating the wound healing process. Overall, this work reveals that metal atom substitution is an effective strategy to construct ultrasmall and high-catalytic-performance PB-based nanozymes and further potentiate their effectiveness for chronic wound management.
由于氧化微环境的存在，慢性伤口愈合仍然具有挑战性。具有多种抗氧化酶样活性的普鲁士蓝（PB）纳米颗粒已引起广泛关注，但其抗氧化功效仍不尽人意。在此，通过简单的方法高产率构建了超小富钙普鲁士蓝纳米颗粒（CaPB NPs）用于伤口修复应用。由于超小尺寸和所产生的双活性位点的协同效应，CaPB NPs表现出显著的抗氧化酶样活性，保护细胞免受氧化应激诱导的损伤。除了钙对调节角质形成细胞和成纤维细胞生长的作用外，已证明在小鼠全层伤口缺损模型中，给予CaPB NPs明显促进了伤口闭合以及胶原蛋白沉积和新血管形成。重要的是，CaPB NP治疗可有效上调抗炎细胞因子和血管内皮生长因子的表达水平，重塑伤口微环境，从而加速伤口愈合过程。总体而言，这项工作表明金属原子取代是构建超小且具有高催化性能的基于PB的纳米酶并进一步增强其对慢性伤口管理有效性的有效策略。
