O'Leary T J, Ross P D, Levin I W
Biophys J. 1986 Dec;50(6):1053-9. doi: 10.1016/S0006-3495(86)83549-4.
The effects of cis- and trans-9,10-tetradecenols on the phase transitions of dimyristoyl-, dipalmitoyl-, and distearoyl-phosphatidylcholines were investigated using high sensitivity scanning calorimetry and Raman spectroscopy. Both alcohols lowered the gel to liquid crystalline phase transition temperatures for all three phosphatidylcholines, with cis-tetradecenol showing a considerably greater effect than trans-tetradecenol in each case. While both alcohols increased the temperature of the dimyristoylphosphatidylcholine pretransition, and decreased the temperature of the distearoylphosphatidylcholine pretransition, cis-tetradecenol lowered the temperature of the dipalmitoylphosphatidylcholine pretransition, while trans-tetradecenol dramatically raised the pretransition temperature. These results are interpreted in terms of the reduction in gel (L beta) phase chain tilt and changes in the ease of acyl chain trans-gauche isomerization which are introduced by the alcohols, and the consequent effects of these changes on the pretransition and the gel to liquid crystalline phase transition. The data clearly show that caution is necessary in applying information on lipid-anesthetic interactions obtained from model membranes to the problem of clinical anesthesia, since qualitatively different results may be obtained when lipids of differing acyl chain lengths are employed. Superficial interpretation of such data might lead to erroneous conclusions.
使用高灵敏度扫描量热法和拉曼光谱法研究了顺式和反式-9,10-十四碳烯醇对二肉豆蔻酰基、二棕榈酰基和二硬脂酰基磷脂酰胆碱相变的影响。两种醇均降低了所有三种磷脂酰胆碱从凝胶相到液晶相的转变温度，在每种情况下，顺式十四碳烯醇的影响都比反式十四碳烯醇大得多。虽然两种醇都提高了二肉豆蔻酰基磷脂酰胆碱预转变的温度，并降低了二硬脂酰基磷脂酰胆碱预转变的温度，但顺式十四碳烯醇降低了二棕榈酰基磷脂酰胆碱预转变的温度，而反式十四碳烯醇则显著提高了预转变温度。这些结果可根据凝胶（Lβ）相链倾斜度的降低以及醇引入的酰基链反式-顺式异构化难易程度的变化，以及这些变化对预转变和凝胶到液晶相转变的后续影响来解释。数据清楚地表明，将从模型膜获得的脂质-麻醉剂相互作用信息应用于临床麻醉问题时必须谨慎，因为使用不同酰基链长度的脂质时可能会得到性质不同的结果。对这些数据的肤浅解释可能会导致错误的结论。
