荷花（Nelumbo nucifera Gaertn）的 NnWOX1-1、NnWOX4-3 和 NnWOX5-1 促进根的形成并增强转基因拟南芥的胁迫耐受性。

NnWOX1-1, NnWOX4-3, and NnWOX5-1 of lotus (Nelumbo nucifera Gaertn)promote root formation and enhance stress tolerance in transgenic Arabidopsis thaliana.

机构信息

College of Horticulture and landscape Architechture, Yangzhou University, Jiangsu, People's Republic of China.

College of Guangling, Yangzhou University, Jiangsu, People's Republic of China.

出版信息

BMC Genomics. 2023 Nov 28;24(1):719. doi: 10.1186/s12864-023-09772-w.

DOI:10.1186/s12864-023-09772-w

PMID:38017402

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10683310/

Abstract

BACKGROUND

Adventitious roots (ARs) represent an important organ system for water and nutrient uptake in lotus plants because of degeneration of the principal root. The WUSCHEL-related homeobox (WOX) gene regulates plant development and growth by affecting the expression of several other genes. In this study, three WOX genes, NnWOX1-1, NnWOX4-3, and NnWOX5-1, were isolated and their functions were assessed in Arabidopsis plants.

RESULTS

The full lengths of NnWOX1-1, NnWOX4-3, and NnWOX5-1 were 1038, 645, and 558 bp, encoding 362, 214, and 185 amino acid residues, respectively. Phylogenetic analysis classified NnWOX1-1 and NnWOX4-3 encoding proteins into one group, and NnWOX5-1 and MnWOX5 encoding proteins exhibited strong genetic relationships. The three genes were induced by sucrose and indoleacetic acid (IAA) and exhibited organ-specific expression characteristics. In addition to improving root growth and salt tolerance, NnWOX1-1 and NnWOX4-3 promoted stem development in transgenic Arabidopsis plants. A total of 751, 594, and 541 genes, including 19, 19, and 13 respective genes related to ethylene and IAA metabolism and responses, were enhanced in NnWOX1-1, NnWOX4-3, and NnWOX5-1 transgenic plants, respectively. Further analysis showed that ethylene production rates in transgenic plants increased, whereas IAA, peroxidase, and lignin content did not significantly change. Exogenous application of ethephon on lotus seedlings promoted AR formation and dramatically increased the fresh and dry weights of the plants.

CONCLUSIONS

NnWOX1-1, NnWOX4-3, and NnWOX5-1 influence root formation, stem development, and stress adaptation in transgenic Arabidopsis plants by affecting the transcription of multiple genes. Among these, changes in gene expression involving ethylene metabolism and responses likely critically affect the development of Arabidopsis plants. In addition, ethylene may represent an important factor affecting AR formation in lotus seedlings.

摘要

背景

不定根（ARs）是莲植物吸收水分和养分的重要器官系统，因为主根退化。WUSCHEL 相关同源盒（WOX）基因通过影响其他几个基因的表达来调节植物的发育和生长。在这项研究中，分离了三个 WOX 基因，NnWOX1-1、NnWOX4-3 和 NnWOX5-1，并在拟南芥植物中评估了它们的功能。

结果

NnWOX1-1、NnWOX4-3 和 NnWOX5-1 的全长分别为 1038、645 和 558bp，分别编码 362、214 和 185 个氨基酸残基。系统发育分析将 NnWOX1-1 和 NnWOX4-3 编码的蛋白质归为一组，而 NnWOX5-1 和 MnWOX5 编码的蛋白质表现出很强的遗传关系。这三个基因受到蔗糖和吲哚乙酸（IAA）的诱导，并表现出器官特异性表达特征。除了改善根生长和耐盐性外，NnWOX1-1 和 NnWOX4-3 还促进了转基因拟南芥植物的茎发育。NnWOX1-1、NnWOX4-3 和 NnWOX5-1 转基因植物中分别有 751、594 和 541 个基因，包括 19、19 和 13 个分别与乙烯和 IAA 代谢和响应相关的基因，分别增强。进一步分析表明，转基因植物中的乙烯生成率增加，而 IAA、过氧化物酶和木质素含量没有明显变化。在莲苗上施用乙烯利促进了不定根的形成，并显著增加了植物的鲜重和干重。