Department of Periodontology, Institute of Health Sciences, Marmara University, Istanbul, Turkey.
Department of Biochemistry, School of Medicine, Marmara University, Istanbul, Turkey.
Biofouling. 2023 Oct-Nov;39(9-10):980-989. doi: 10.1080/08927014.2023.2288071. Epub 2023 Nov 28.
This study compared the cytotoxicity and antimicrobial activity of hypochlorous acid (HOCl) at 50 ppm and 200 ppm and 0.2% chlorhexidine (CHX) at various time intervals, . Cell viability and cytotoxicity of human gingival fibroblasts (HGF) were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and the lactate dehydrogenase assay. Antimicrobial effects on and were determined using the time-kill method. All solutions exhibited a significant impact on HGFs in a dose- and time-dependent manner. 50 ppm HOCl demonstrated the highest cell viability, followed by 200 ppm HOCl. Both HOCl solutions were less cytotoxic to HGFs than 0.2% CHX. 50 ppm and 200 ppm HOCl demonstrated stronger efficiencies than CHX against and . The data suggest that HOCl solutions have potential as an alternative antiseptic to CHX due to their lower cytotoxicity and superior antimicrobial activity, but optimal dosage of HOCl requires further investigations.
本研究比较了 50ppm 和 200ppm 次氯酸（HOCl）和 0.2%洗必泰（CHX）在不同时间间隔的细胞毒性和抗菌活性。采用 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）试验和乳酸脱氢酶试验评估人牙龈成纤维细胞（HGF）的细胞活力和细胞毒性。采用时间杀伤法测定对 和 的抗菌效果。所有溶液均以剂量和时间依赖的方式对 HGF 产生显著影响。50ppm HOCl 表现出最高的细胞活力，其次是 200ppm HOCl。两种 HOCl 溶液对 HGF 的细胞毒性均低于 0.2%CHX。50ppm 和 200ppm HOCl 对 和 的杀菌效果均强于 CHX。数据表明，由于 HOCl 溶液的细胞毒性较低，抗菌活性较高，因此具有替代 CHX 的潜力，但 HOCl 的最佳剂量需要进一步研究。
