Takeda K, Shimahara T, Tauc L, Berwald-Netter Y
Brain Res. 1986 Nov 19;398(1):75-84. doi: 10.1016/0006-8993(86)91252-7.
The nicotine and muscarinic responses of differentiated mouse neuroblastoma cells from the clonal line N1E 115 to applied cholinergic agents were recorded using single channel and whole cell patch clamp techniques. An inward macroscopic current induced by acetylcholine (ACh) at the resting potential was blocked by curare; cell-attached recordings revealed a single channel conductance of 18 pS and a lifetime of 36 ms at 30 degrees C, with 200 nM ACh. The zero current potential was close to 0 mV. The kinetics of these nicotinic currents were described by multiexponential functions for both the open and closed time distributions. An outward single channel current, present at resting and slightly depolarized potentials, was also observed and has been tentatively described as being dependent on muscarinic receptor activation, as it was usually blocked by atropine. Under our conditions of whole cell clamp, no macroscopic outward current sensitive to ACh was observed.
使用单通道和全细胞膜片钳技术记录了克隆系N1E 115分化的小鼠神经母细胞瘤细胞对应用的胆碱能药物的尼古丁和毒蕈碱反应。在静息电位下，乙酰胆碱（ACh）诱导的内向宏观电流被箭毒阻断；细胞贴附记录显示，在30℃时，200 nM ACh作用下，单通道电导为18 pS，寿命为36 ms。零电流电位接近0 mV。这些烟碱电流的动力学由开放和关闭时间分布的多指数函数描述。还观察到在静息和轻度去极化电位下存在的外向单通道电流，初步描述为依赖于毒蕈碱受体激活，因为它通常被阿托品阻断。在我们的全细胞钳制条件下，未观察到对ACh敏感的宏观外向电流。
