West Kazakhstan Marat Ospanov University, Aktobe, Kazakhstan.
Astana Medical University, Astana, Kazakhstan.
Asian Pac J Cancer Prev. 2023 Nov 1;24(11):3925-3930. doi: 10.31557/APJCP.2023.24.11.3925.
Hepatitis C virus (HCV) infection is a common cause of cirrhosis worldwide, leading to significant economic and social burdens. Approximately 170 million people (3% of the population) are infected with HCV, with the risk of developing complications such as cirrhosis and hepatocellular carcinoma. In the United States, HCV is the main cause of liver cirrhosis, accounting for 26% of cases. Recent studies have shown an increase in the proportion of HCV-related liver cirrhosis.
A total of 102 patients with chronic hepatitis C in the reactivation phase from the Atyrau and Aktobe regional hepatology centers, who had not previously received antiviral therapy, were examined. A control group, matched by gender and age, included 127 practically healthy individuals of Kazakh nationality. All patients underwent a comprehensive examination, which included a complete blood count, a biochemical blood analysis and PCR for HCV. Venous blood samples were taken from all subjects for molecular genetic analysis. Genotyping of TLR3 polymorphism (rs5743312, rs5743305, rs3775291, rs5743311, rs1879026) was performed using real-time PCR. Thes study is a case control study.
In patients with cirrhosis of the liver resulting from chronic hepatitis C (HCV), the results of biochemical analysis were statistically significantly higher than in patients with HCV without liver cirrhosis: the levels of total bilirubin (p 0.017*), alkaline phosphatase (p 0.022*), and gamma-glutamyl transferase (0.041*) were elevated. The results indicated that the CC genotype of TLR3 rs1879026 was associated with the development and chronicity of HCV infection compared to practically healthy individuals (p=0.001). In the distribution of genotypes and alleles for rs5743312, rs5743305, rs3775291, and rs5743311, no significant differences were found between patients with HCV and the healthy control group.
The TLR3 rs1879026 gene polymorphism plays a significant role in the predisposition to HCV infection in the Kazakh population of the Aktobe and Atyrau regions.
丙型肝炎病毒（HCV）感染是全球肝硬化的常见病因，给经济和社会带来了巨大的负担。全球约有 1.7 亿人（占人口的 3%）感染 HCV，存在发展为肝硬化和肝细胞癌等并发症的风险。在美国，HCV 是肝硬化的主要病因，占 26%。最近的研究表明，与 HCV 相关的肝硬化比例有所增加。
共检查了来自阿特劳和阿克托比地区肝病中心的 102 例慢性丙型肝炎再激活期未接受抗病毒治疗的患者。对照组由 127 名哈萨克族、性别和年龄匹配的健康个体组成。所有患者均接受了全面检查，包括全血细胞计数、生化血液分析和 HCV-PCR。所有受试者均抽取静脉血进行分子遗传学分析。采用实时 PCR 法检测 TLR3 多态性（rs5743312、rs5743305、rs3775291、rs5743311、rs1879026）的基因分型。本研究为病例对照研究。
慢性丙型肝炎（HCV）导致的肝硬化患者的生化分析结果明显高于无肝硬化的 HCV 患者：总胆红素水平升高（p<0.017*）、碱性磷酸酶（p<0.022*）和γ-谷氨酰转移酶（0.041*）。结果表明，与健康个体相比，TLR3 rs1879026 的 CC 基因型与 HCV 的发生和慢性化有关（p=0.001）。rs5743312、rs5743305、rs3775291 和 rs5743311 的基因型和等位基因分布在 HCV 患者和健康对照组之间无显著差异。
TLR3 rs1879026 基因多态性在阿克托比和阿特劳地区哈萨克人群中对 HCV 感染的易感性有重要作用。
