Robinson M, Laurie R D, Bull R J, Stober J A
Cancer Lett. 1987 Jan;34(1):49-54. doi: 10.1016/0304-3835(87)90072-3.
Coal tar paints are among the products used as inside coatings for water pipes and storage tanks to retard corrosion in potable water supply systems. Four different formulations of these paints were tested in earlier work by this laboratory in the Ames mutagenesis and the mouse skin carcinogenesis bioassays. The paint most active in these assays were then tested in a particulate form in the lung adenoma assay with A/J mice. The paint was applied to clean glass plates, cured, collected and homogenized in 2% Emulphor. Doses of this coal tar suspension were administered by gavage at 1.0, 10.0 and 55.0 mg in 0.2 ml per mouse 3X weekly for 8 weeks. The total doses of coal tar paint were 24, 240, and 1320 mg/mouse. Benzo[a]pyrene (BaP), administered in a parallel schedule to a total dose of 6 mg/mouse, served as positive control. A negative control group received an equivalent volume of 2% Emulphor. Animals were killed at 9 months of age (8 months after first dose) and lung adenomas counted. A dose-related response, in the average number of lung tumors per mouse, was observed with the coal tar particulate. There were also squamous cell tumors of the forestomach in 42% of the mice receiving 55.0 mg coal tar paint per application.
煤焦油涂料是用于水管和储水箱内部涂层以延缓饮用水供应系统腐蚀的产品之一。本实验室在早期工作中对这些涂料的四种不同配方进行了艾姆斯诱变试验和小鼠皮肤致癌生物测定。然后，在这些试验中活性最高的涂料以颗粒形式在A/J小鼠的肺腺瘤试验中进行测试。将涂料涂覆在干净的玻璃板上，固化后收集并在2%乳化剂中匀浆。以每只小鼠0.2毫升含1.0、10.0和55.0毫克的剂量通过灌胃给药该煤焦油悬浮液，每周3次，共8周。煤焦油涂料的总剂量分别为每只小鼠24、240和1320毫克。苯并[a]芘（BaP）按照平行方案给药，总剂量为每只小鼠6毫克，作为阳性对照。阴性对照组接受等量体积的2%乳化剂。动物在9个月龄（首次给药后8个月）处死并计数肺腺瘤。观察到煤焦油颗粒导致每只小鼠肺肿瘤平均数量呈剂量相关反应。在每次接受55.0毫克煤焦油涂料的小鼠中，也有42%出现了前胃鳞状细胞瘤。
