兔和豚鼠心脏中前列环素依赖性冠状动脉舒张

Prostacyclin-dependent coronary vasodilation in rabbit and guinea pig hearts.

作者信息

Wennmalm A

出版信息

Acta Physiol Scand. 1979 May;106(1):47-52. doi: 10.1111/j.1748-1716.1979.tb06368.x.

DOI:10.1111/j.1748-1716.1979.tb06368.x

Abstract

Isolated hearts from rabbits or guinea pigs were perfused according to Langendorff and the coronary flow was recorded continuously. In addition, the rabbit heart transmyocardial effluent's content of platelet anti-aggregatory (prostacyclin-like, PCLA) activity was assayed biologically at regular intervals. Perfusion was performed with a solution gassed with 95% O2 in CO2, switching at intervals to a solution gassed with 12% O2 and 5% CO2 in N2. Perfusion with a hypoxic solution elicited reproducible increased in coronary flow. After pretreatment with indomethacin (5 x 10-5M), this increase was completely abolished and in several cases it was reversed to a marked reduction in cornonary flow. The transmyocardial effluent contained, during perfusion with normoxic solution, no detectable or only negligible amounts of PCLA. During hypoxia the efflux of PCAL into the transmyocardial effluent increased markedly. This increase was completely abolished when indomethacin (5 x 10-5M) was added to the solution perfusing the heart. The results strongly suggest that increased coronary vascular formation of prostacyclin plays a key role in the coronary vasodilation induced by hypoxia in rabbit and guinea pig hearts.

摘要

按照Langendorff法对兔或豚鼠的离体心脏进行灌注，并持续记录冠脉流量。此外，定期对兔心脏心肌跨膜流出液中血小板抗聚集（类前列环素，PCLA）活性的含量进行生物学测定。用含95%氧气和二氧化碳的混合气体的溶液进行灌注，每隔一段时间切换为含12%氧气、5%二氧化碳和氮气的混合气体的溶液。用低氧溶液灌注可引起冠脉流量重复性增加。用吲哚美辛（5×10⁻⁵M）预处理后，这种增加完全消失，在某些情况下反而导致冠脉流量显著减少。在常氧溶液灌注期间，心肌跨膜流出液中未检测到或仅含有可忽略不计的PCLA量。在低氧期间，PCAL向心肌跨膜流出液中的流出显著增加。当向灌注心脏的溶液中加入吲哚美辛（5×10⁻⁵M）时，这种增加完全消失。结果强烈表明，前列环素冠脉血管生成增加在兔和豚鼠心脏低氧诱导的冠脉血管舒张中起关键作用。