Zhang Wuwen, Li Kai, Li Shifeng, Lv Rong, Ma Jie, Yin Ping, Li Li, Sun Ningyu, Chen Yuanyuan, Lu Lu, Li Yun, Zhang Qinhua, Yan Hua
Reproductive Medicine Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.
Front Physiol. 2023 Nov 8;14:1279559. doi: 10.3389/fphys.2023.1279559. eCollection 2023.
Many clinical studies have shown that embryos of fertilization (IVF) are often prone to developmental arrest, which leads to recurrent failure of IVF treatment. Early embryonic arrest has always been an urgent clinical problem in assisted reproduction centers. However, the molecular mechanisms underlying early embryonic development arrest remain largely unknown. The objective of this study is to investigate potential candidate hub genes and key signaling pathways involved in early stages of embryonic development. RNA-seq analysis was performed on normal and arrest embryos to study the changes of gene expression during early embryonic development. A total of 520 genes exhibiting differential expression were identified, with 174 genes being upregulated and 346 genes being downregulated. Upregulated genes show enrichment in biosynthesis, cellular proliferation and differentiation, and epigenetic regulation. While downregulated genes exhibit enrichment in transcriptional activity, epigenetic regulation, cell cycle progression, cellular proliferation and ubiquitination. The STRING (search tool for the retravel of interacting genes/proteins) database was utilized to analyze protein-protein interactions among these genes, aiming to enhance comprehension of the potential role of these differentially expressed genes (DEGs). A total of 22 hub genes (highly connected genes) were identified among the DEGs using Cytoscape software. Of these, and were upregulated, while the remaining 20 genes ( and ) were downregulated. These hub genes are associated with crucial biological processes such as ubiquitination, cellular senescence, cell proliferation and differentiation, and cell cycle. Among these hub genes, and may be involved in controlling cell cycle, which are critical process in early embryonic development.
许多临床研究表明，体外受精（IVF）胚胎常常易于发生发育停滞，这导致IVF治疗反复失败。早期胚胎停滞一直是辅助生殖中心亟待解决的临床问题。然而，早期胚胎发育停滞背后的分子机制仍 largely unknown。本研究的目的是调查参与胚胎发育早期阶段的潜在候选枢纽基因和关键信号通路。对正常和停滞胚胎进行RNA测序分析，以研究早期胚胎发育过程中基因表达的变化。共鉴定出520个表现出差异表达的基因，其中174个基因上调，346个基因下调。上调基因在生物合成、细胞增殖与分化以及表观遗传调控方面表现出富集。而下调基因在转录活性、表观遗传调控、细胞周期进程、细胞增殖和泛素化方面表现出富集。利用STRING（相互作用基因/蛋白质检索工具）数据库分析这些基因之间的蛋白质-蛋白质相互作用，旨在增强对这些差异表达基因（DEGs）潜在作用的理解。使用Cytoscape软件在DEGs中总共鉴定出22个枢纽基因（高度连接的基因）。其中，[此处基因名称缺失]上调，而其余20个基因（[此处基因名称缺失]）下调。这些枢纽基因与泛素化、细胞衰老、细胞增殖与分化以及细胞周期等关键生物学过程相关。在这些枢纽基因中，[此处基因名称缺失]可能参与控制细胞周期，这是早期胚胎发育中的关键过程。
