Zhang Wenshuo, Zhang Hairong, Li Jiaxin, Zou Xiangqing, Wang Wanru, Hu Haiyan, Iqbal Kanwal, Zhou Panpan, Ye Weichun
State Key Laboratory of Applied Organic Chemistry and Department of Chemistry, Lanzhou University, Lanzhou, 730000, China.
Key Laboratory of Special Functional Materials and Structural Design, Ministry of Education, Lanzhou University, Lanzhou, 730000, China.
Mikrochim Acta. 2023 Dec 2;191(1):1. doi: 10.1007/s00604-023-06047-9.
A polyvinylpyrrolidone-capped (PVP-capped) strategy is reported to synthesize Ag NPs on silicon wafers via galvanic replacement reaction for SERS detection of adenine, where PVP acts as stabilizing agent in synthesis and efficient enrichment in detection. The morphologies of Ag NPs are optimized with uniform particle size by adjusting synthesis conditions, which hold excellent SERS performances like a high enhancement factor of 1.42 × 10, good uniform, reproducibility, and transferable nature. With the protection of the capped PVP, the Ag NPs keep excellent SERS properties even against harsh conditions of high temperature (100 ℃) and strong acid and base for 24 h. Utilizing the structural feature of PVP with abundant carbonyl groups, the PVP-capped Ag NPs achieve efficient enrichment of adenine through hydrogen bonding and π-interactions, which is analyzed by density functional theory. Quantitative detection of adenine is performed with a wide linear range from 10 to 10 M and a low limit of detection of 1 nM. Detection of adenine in human urine samples is achieved with a recovery of 99.1-103.4% and an RSD of less than 5%.
据报道，一种聚乙烯吡咯烷酮包覆（PVP包覆）策略可通过电置换反应在硅片上合成银纳米颗粒（Ag NPs），用于腺嘌呤的表面增强拉曼光谱（SERS）检测，其中PVP在合成过程中作为稳定剂，并在检测中实现高效富集。通过调整合成条件优化了Ag NPs的形态，使其粒径均匀，具有优异的SERS性能，如高达1.42×10的高增强因子、良好的均匀性、重现性和可转移特性。在包覆的PVP保护下，即使在高温（100℃）和强酸强碱的苛刻条件下暴露24小时，Ag NPs仍保持优异的SERS性能。利用PVP具有丰富羰基的结构特征，PVP包覆的Ag NPs通过氢键和π相互作用实现了腺嘌呤的高效富集，这一点通过密度泛函理论进行了分析。腺嘌呤的定量检测线性范围宽，为10至10 M，检测下限低至1 nM。在人尿样中检测腺嘌呤，回收率为99.1 - 103.4%，相对标准偏差小于5%。
