Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China.
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China.
J Hazard Mater. 2024 Mar 5;465:133029. doi: 10.1016/j.jhazmat.2023.133029. Epub 2023 Nov 18.
Tracking and imaging of nano-plastics are extremely challenging, especially in fresh biological samples. Here, we propose a new strategy in which polystyrene (PS) was doped with the europium chelate Eu (DBM)bpy to quantify, track, and in situ image nano-plastics in fresh cucumber based on inherent metals using cryogenic laser ablation inductively coupled plasma mass spectrometry (cryo-LA-ICP-MS). The cryogenic conditions provide a stable condition for imaging fresh cucumber, suppressing the evaporation of water in fresh plants, and maintaining the original structure of plants with respect to room temperature imaging in LA-ICP-MS. The plants were cultivated in two types of nano-plastics solutions with low (50 mg/L) and high (200 mg/L) concentrations for 9 days. The results showed that nano-plastics mainly enrich the roots and have negative effects, which decrease the trace elements of Zn, Mn, and Cu in cucumber. Smaller PS particles are able to penetrate the plant more easily and inflict serious damage. Novel imaging method provides a novel insight into the tracking and imaging of nano-plastics in fresh plant samples. The results illustrated that nano-plastics deposition on plants has the potential to have direct ecological effects as well as consequences for potential health.
纳米塑料的追踪和成像极具挑战性，特别是在新鲜生物样本中。在这里，我们提出了一种新策略，即在聚苯乙烯（PS）中掺杂铕螯合物 Eu（DBM）bpy，利用低温激光烧蚀电感耦合等离子体质谱法（cryo-LA-ICP-MS），基于固有金属定量、追踪和原位成像新鲜黄瓜中的纳米塑料。低温条件为成像新鲜黄瓜提供了稳定的条件，抑制了新鲜植物中水分的蒸发，并保持了植物的原始结构，相对于 LA-ICP-MS 室温成像。这些植物在两种浓度分别为低（50mg/L）和高（200mg/L）的纳米塑料溶液中培养了 9 天。结果表明，纳米塑料主要在根部富集并产生负面影响，导致黄瓜中锌、锰和铜等微量元素减少。较小的 PS 颗粒更容易穿透植物并造成严重损害。新的成像方法为追踪和成像新鲜植物样本中的纳米塑料提供了新的视角。结果表明，纳米塑料在植物上的沉积有可能对潜在的健康产生直接的生态影响。
