• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

二氧化铈纳米颗粒对再生水-土壤-萝卜系统中抗生素抗性传播的影响——中国广东省深圳市，2023年4月

Effect of CeO Nanoparticles on the Spread of Antibiotic Resistance in a Reclaimed Water-Soil-Radish System - Shenzhen City, Guangdong Province, China, April 2023.

作者信息

Yu Kaiqiang, Zhao Bixi, Yan Yuxi, Yang Qing, Chen Liming, Xia Yu

机构信息

School of Environmental Science and Engineering, College of Engineering, Southern University of Science and Technology, Shenzhen City, Guangdong Province, China.

School of Resource, Environment and Life Science, Ningxia Normal University, Guyuan City, Gansu Province, China.

出版信息

China CDC Wkly. 2023 Nov 17;5(46):1029-1037. doi: 10.46234/ccdcw2023.194.

DOI:10.46234/ccdcw2023.194
PMID:38046641
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10689965/
Abstract

INTRODUCTION

The use of reclaimed water (RW) for irrigation in agricultural practices raises concerns regarding the dissemination of antibiotic resistance genes (ARGs) from soils to edible crops. The effectiveness of nanoparticles (NPs) in reducing antibiotic resistance in vegetables irrigated with RW remains largely unexplored.

METHODS

To investigate the effects, we conducted pot experiments in which radishes were planted in soil amended with CeO NPs using various application techniques. The abundance of ARGs was characterized using high-throughput quantitative PCR (HT-qPCR). Concurrently, we utilized 16S ribosomal RNA (rRNA) gene sequencing to evaluate the microbial community structure of both the rhizosphere soil and the endophytic compartment within the radishes. Employing bioinformatics analysis, we probed the potential mechanisms by which NPs influence the resistome within the reclaimed water-soil-radish system.

RESULTS

Following the application of CeO NPs, there was a noticeable reduction in both the number and concentration of ARG genotypes in the rhizosphere soil, as well as within the radish. Concurrently, CeO NPs appeared to mitigate the propagation of ARGs within the reclaimed water-soil-radish system. The ability of CeO NPs to modulate the resistome is linked to alterations in microbial community structure. Soil treatment with NPs emerged as the most effective strategy for curbing the spread of ARGs.

DISCUSSION

This finding provides a theoretical foundation for the development of nano-agricultural technologies aimed at controlling the proliferation of ARGs.

摘要

引言

在农业实践中使用再生水（RW）进行灌溉引发了人们对土壤中抗生素抗性基因（ARG）传播到可食用作物的担忧。纳米颗粒（NPs）在降低用RW灌溉的蔬菜中抗生素抗性方面的有效性在很大程度上仍未得到探索。

方法

为了研究其效果，我们进行了盆栽实验，其中使用各种施用技术将萝卜种植在用CeO NPs改良的土壤中。使用高通量定量PCR（HT-qPCR）对ARG的丰度进行表征。同时，我们利用16S核糖体RNA（rRNA）基因测序来评估根际土壤和萝卜内生区室的微生物群落结构。通过生物信息学分析，我们探究了NPs影响再生水-土壤-萝卜系统中抗性组的潜在机制。

结果

施用CeO NPs后，根际土壤以及萝卜中ARG基因型的数量和浓度均显著降低。同时，CeO NPs似乎减轻了ARG在再生水-土壤-萝卜系统中的传播。CeO NPs调节抗性组的能力与微生物群落结构的改变有关。用NPs处理土壤是抑制ARG传播的最有效策略。

讨论

这一发现为开发旨在控制ARG扩散的纳米农业技术提供了理论基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/fa7c1fe956b5/ccdcw-5-46-1029-S4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/fe2853c74a64/ccdcw-5-46-1029-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/502e35091739/ccdcw-5-46-1029-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/2c09e0c4bfff/ccdcw-5-46-1029-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/aec6c3b170b7/ccdcw-5-46-1029-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/07f32600f488/ccdcw-5-46-1029-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/43a946c95e31/ccdcw-5-46-1029-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/601ba9a6f588/ccdcw-5-46-1029-S1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/b2cb40106a94/ccdcw-5-46-1029-S2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/a1946f6447b7/ccdcw-5-46-1029-S3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/fa7c1fe956b5/ccdcw-5-46-1029-S4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/fe2853c74a64/ccdcw-5-46-1029-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/502e35091739/ccdcw-5-46-1029-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/2c09e0c4bfff/ccdcw-5-46-1029-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/aec6c3b170b7/ccdcw-5-46-1029-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/07f32600f488/ccdcw-5-46-1029-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/43a946c95e31/ccdcw-5-46-1029-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/601ba9a6f588/ccdcw-5-46-1029-S1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/b2cb40106a94/ccdcw-5-46-1029-S2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/a1946f6447b7/ccdcw-5-46-1029-S3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78ac/10689965/fa7c1fe956b5/ccdcw-5-46-1029-S4.jpg

相似文献

1
Effect of CeO Nanoparticles on the Spread of Antibiotic Resistance in a Reclaimed Water-Soil-Radish System - Shenzhen City, Guangdong Province, China, April 2023.二氧化铈纳米颗粒对再生水-土壤-萝卜系统中抗生素抗性传播的影响——中国广东省深圳市，2023年4月
China CDC Wkly. 2023 Nov 17;5(46):1029-1037. doi: 10.46234/ccdcw2023.194.
2
Dissemination of antimicrobial resistance in agricultural ecosystems following irrigation with treated municipal wastewater.经处理后的城市污水灌溉对农业生态系统中抗药性传播的影响。
Sci Total Environ. 2024 Jul 15;934:173288. doi: 10.1016/j.scitotenv.2024.173288. Epub 2024 May 18.
3
Effects of nitrogen fertilization on the fate of high-risk antibiotic resistance genes in reclaimed water-irrigated soil and plants.氮肥对再生水灌溉土壤和植物中高风险抗生素耐药基因命运的影响。
Environ Int. 2024 Aug;190:108834. doi: 10.1016/j.envint.2024.108834. Epub 2024 Jun 18.
4
Metagenomics-assembled analysis revealed the characteristics of antibiotic resistome and community coalescence in the soils irrigated with different irrigation materials.宏基因组组装分析揭示了不同灌溉物料灌溉土壤中抗生素抗性组和群落凝聚的特征。
Sci Total Environ. 2024 Jan 10;907:167868. doi: 10.1016/j.scitotenv.2023.167868. Epub 2023 Oct 15.
5
Cropping system exerts stronger influence on antibiotic resistance gene assemblages in greenhouse soils than reclaimed wastewater irrigation.轮作制度对温室土壤中抗生素抗性基因组合的影响大于再生水灌溉。
J Hazard Mater. 2022 Mar 5;425:128046. doi: 10.1016/j.jhazmat.2021.128046. Epub 2021 Dec 10.
6
Amendment soil with biochar to control antibiotic resistance genes under unconventional water resources irrigation: Proceed with caution.用生物炭改良土壤以控制非常规水资源灌溉下的抗生素抗性基因：谨慎行事。
Environ Pollut. 2018 Sep;240:475-484. doi: 10.1016/j.envpol.2018.04.143. Epub 2018 May 11.
7
Impacts of reclaimed water irrigation on soil antibiotic resistome in urban parks of Victoria, Australia.回收水灌溉对澳大利亚维多利亚州城市公园土壤抗生素抗性组的影响。
Environ Pollut. 2016 Apr;211:48-57. doi: 10.1016/j.envpol.2015.12.033. Epub 2015 Dec 29.
8
Flooding drives the temporal turnover of antibiotic resistance gene in manure-amended soil-water continuum.洪水驱动了抗生素抗性基因在粪肥添加土壤-水连续体中的时间转换。
Environ Int. 2023 Sep;179:108168. doi: 10.1016/j.envint.2023.108168. Epub 2023 Aug 23.
9
[Effects of Soil Amendments on the Bacterial Diversity and Abundances of Pathogens and Antibiotic Resistance Genes in Rhizosphere Soil Under Drip Irrigation with Reclaimed Water].[土壤改良剂对再生水滴灌条件下根际土壤中细菌多样性、病原菌丰度及抗生素抗性基因的影响]
Huan Jing Ke Xue. 2022 Oct 8;43(10):4765-4778. doi: 10.13227/j.hjkx.202111267.
10
Insights into antibiotic resistance-related changes in microbial communities, resistome and mobilome in paddy irrigated with reclaimed wastewater.探讨再生水灌溉稻田中微生物群落、抗药基因库和可移动遗传元件的抗生素耐药性相关变化。
Sci Total Environ. 2023 Nov 20;900:165672. doi: 10.1016/j.scitotenv.2023.165672. Epub 2023 Jul 20.

本文引用的文献

1
Interplay of Biologically Active Carbon Filtration and Chlorine-Based Disinfection in Mitigating the Dissemination of Antibiotic Resistance Genes in Water Reuse Distribution Systems.生物活性炭过滤与基于氯的消毒在减轻再生水分配系统中抗生素耐药基因传播中的相互作用。
Environ Sci Technol. 2021 Jun 15;55(12):8329-8340. doi: 10.1021/acs.est.1c01199. Epub 2021 Jun 3.
2
Two chemically distinct root lignin barriers control solute and water balance.两种化学性质不同的根木质素屏障控制溶质和水分平衡。
Nat Commun. 2021 Apr 19;12(1):2320. doi: 10.1038/s41467-021-22550-0.
3
CeO Nanoparticles Regulate the Propagation of Antibiotic Resistance Genes by Altering Cellular Contact and Plasmid Transfer.
CeO 纳米颗粒通过改变细胞接触和质粒转移来调节抗生素耐药基因的传播。
Environ Sci Technol. 2020 Aug 18;54(16):10012-10021. doi: 10.1021/acs.est.0c01870. Epub 2020 Jul 29.
4
Nano-enabled improvements of growth and nutritional quality in food plants driven by rhizosphere processes.通过根际过程实现的纳米增强型粮食作物生长和营养品质改善。
Environ Int. 2020 Sep;142:105831. doi: 10.1016/j.envint.2020.105831. Epub 2020 Jun 12.
5
Early development of apoplastic barriers and molecular mechanisms in juvenile maize roots in response to LaO nanoparticles.LaO 纳米颗粒诱导幼年玉米根系质外体屏障的早期形成及其分子机制
Sci Total Environ. 2019 Feb 25;653:675-683. doi: 10.1016/j.scitotenv.2018.10.320. Epub 2018 Oct 30.
6
Induction of Escherichia coli into a VBNC state through chlorination/chloramination and differences in characteristics of the bacterium between states.通过氯化/氯胺诱导大肠杆菌进入 VBNC 状态以及状态之间细菌特性的差异。
Water Res. 2018 Oct 1;142:279-288. doi: 10.1016/j.watres.2018.05.055. Epub 2018 May 30.
7
Continental-scale pollution of estuaries with antibiotic resistance genes.抗生素耐药基因在河口的大陆尺度污染。
Nat Microbiol. 2017 Jan 30;2:16270. doi: 10.1038/nmicrobiol.2016.270.
8
Antibiotic resistome and its association with bacterial communities during sewage sludge composting.抗生素耐药组及其在污水污泥堆肥过程中与细菌群落的关系。
Environ Sci Technol. 2015 Jun 16;49(12):7356-63. doi: 10.1021/acs.est.5b01012. Epub 2015 Jun 5.
9
Reproducibility and quantitation of amplicon sequencing-based detection.基于扩增子测序的检测的可重复性和定量分析。
ISME J. 2011 Aug;5(8):1303-13. doi: 10.1038/ismej.2011.11. Epub 2011 Feb 24.
10
Search and clustering orders of magnitude faster than BLAST.比 BLAST 快几个数量级的搜索和聚类。
Bioinformatics. 2010 Oct 1;26(19):2460-1. doi: 10.1093/bioinformatics/btq461. Epub 2010 Aug 12.