Department of Basic and Clinical Sciences, University of Nicosia Medical School, 21 Ilia Papakyriakou, 2414 Engomi, P.O. Box 24005, 1700, Nicosia, Cyprus.
Department of Life Sciences, School of Life and Health Sciences, University of Nicosia, Nicosia, Cyprus.
Acta Parasitol. 2024 Mar;69(1):1035-1040. doi: 10.1007/s11686-023-00747-1. Epub 2023 Dec 8.
Cryptosporidium is an intestinal zoonotic protozoan parasite that infects domesticated and wild animals. There are no reports on the prevalence and molecular characterisation of Cryptosporidium in the endemic Cyprus mouflon. The mouflon is strictly protected by national and international legislation. Its main distribution is Paphos State Forest and surrounding areas, where it may share the same water sources as free-ranging domestic goats. Therefore, the present study aimed to determine the prevalence of Cryptosporidium spp. and genotypes in mouflon and free-ranging goats within the mouflon range.
Faecal samples of 70 mouflons and 34 free-ranging goats were screened for Cryptosporidium by PCR amplification and sequencing.
Only one sample (1/70) belonging to a mouflon was PCR positive for Cryptosporidium. Based on sequencing of the 18S rRNA locus, this species was identified as Cryptosporidium parvum (C. parvum). No positive sample was detected in the free-ranging goats (0/34).
This is the first report on the molecular identification of this Cryptosporidium species in a Cyprus mouflon. The results indicate that the prevalence of Cryptosporidium in Cyprus mouflon is low.
隐孢子虫是一种肠道的动物源性原虫寄生虫，可感染驯养和野生动物。目前尚无有关地方性塞浦路斯摩弗伦羊中隐孢子虫流行情况和分子特征的报道。摩弗伦羊受到国家和国际立法的严格保护。它的主要分布地区是帕福斯州立森林及其周边地区，在那里它可能与自由放养的山羊共享相同的水源。因此，本研究旨在确定摩弗伦羊和摩弗伦羊范围内自由放养山羊中隐孢子虫属和基因型的流行情况。
用 PCR 扩增和测序法对 70 只摩弗伦羊和 34 只自由放养山羊的粪便样本进行隐孢子虫筛查。
只有一只摩弗伦羊（1/70）的 PCR 结果呈隐孢子虫阳性。根据 18S rRNA 基因座的测序结果，该物种被鉴定为小隐孢子虫（C. parvum）。在自由放养的山羊中未检测到阳性样本（0/34）。
这是首次在塞浦路斯摩弗伦羊中对这种隐孢子虫种进行分子鉴定的报告。结果表明，塞浦路斯摩弗伦羊中隐孢子虫的流行率较低。
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