塞浦路斯家养反刍动物和野生爬行动物中隐孢子虫种的分子鉴定。
Molecular identification of Cryptosporidium species from domestic ruminants and wild reptiles in Cyprus.
机构信息
Department of Basic and Clinical Sciences, University of Nicosia Medical School, 21 Ilia Papakyriakou, 2414 Engomi, P.O. Box 24005, 1700, Nicosia, Cyprus.
出版信息
Parasitol Res. 2022 Jul;121(7):2193-2198. doi: 10.1007/s00436-022-07527-2. Epub 2022 Apr 30.
The presence of Cryptosporidium species in faecal samples of 32 sheep (Aries bovis), 10 goats (Capra circus), 1 blunt nose viper (Macrovipera lebetina lebetina), 3 Kotschy's geckos (Mediodactylus kotschyi) and 6 wild stellagamas (Stellagama stellio cypriaca) in Cyprus were investigated by polymerase chain reaction (PCR) and sequencing. Cryptosporidium species were found in 9/32 sheep, 5/10 goats, 2/3 Kotschy's geckos and 2/6 stellagamas faecal samples based on the sequencing of the 18S rRNA gene. Subtyping was achieved based on the sequencing of the gp60 gene. Four different species have been identified: Cryptosporidium parvum in goats (subtype IIaA15G1R1), C. xiaoi (subtypes XXIIId and XXIIIl) and C. ubiquitum (subtype XIIa) in sheep and C. varanii and C. parvum in lizards; the viper snake sample was negative. This is the first report on the molecular identification of a variety of Cryptosporidium species from domestic ruminants and wild reptiles in the Republic of Cyprus.
通过聚合酶链反应（PCR）和测序的方法，对塞浦路斯的 32 只绵羊（牛属）、10 只山羊（山羊属）、1 条钝鼻蝰蛇（钝鼻蝰属）、3 只科氏壁虎（科氏壁虎属）和 6 只野生菱斑鳖蜥（菱斑鳖蜥属）的粪便样本中的隐孢子虫种类进行了调查。根据 18S rRNA 基因的测序结果，在 9/32 只绵羊、5/10 只山羊、2/3 只科氏壁虎和 2/6 只菱斑鳖蜥粪便样本中发现了隐孢子虫。通过对 gp60 基因的测序实现了亚型分型。鉴定出了 4 种不同的物种：山羊中的微小隐孢子虫（亚型 IIaA15G1R1）、绵羊中的小隐孢子虫（XXIIId 和 XXIIIl 亚型）和 C. ubiquitum（XIIa 亚型）以及 C. varanii 和 C. parvum （小隐孢子虫）在蜥蜴中；蝰蛇样本为阴性。这是首次在塞浦路斯共和国从家养反刍动物和野生爬行动物中分子鉴定出多种隐孢子虫的报告。
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