Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drug, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, China.
BMC Genomics. 2023 Dec 7;24(1):754. doi: 10.1186/s12864-023-09850-z.
Bergenia purpurascens is an important medicinal, edible and ornamental plant. It generally grows in high-altitude areas with complex climates. There have been no reports about how B. purpurascens survives under cold stress. Here, the B. purpurascens under low temperature were subjected to transcriptomics analysis to explore the candidate genes and pathways that involved in the cold tolerance of B. purpurascens. Compared with the control treatment, we found 9,600 up-regulated differentially expressed genes (DEGs) and 7,055 down-regulated DEGs. A significant number of DEGs were involved in the Ca signaling pathway, mitogen-activated protein kinase (MAPK) cascade, plant hormone signaling pathway, and lipid metabolism. A total of 400 transcription factors were found to respond to cold stress, most of which belonged to the MYB and AP2/ERF families. Five novel genes were found to be potential candidate genes involved in the cold tolerance of B. purpurascens. The study provide insights into further investigation of the molecular mechanism of how B. purpurascens survives under cold stress.
宝兴百合是一种重要的药用、食用和观赏植物。它通常生长在气候复杂的高海拔地区。目前还没有关于宝兴百合如何在寒冷胁迫下生存的报道。本研究对低温胁迫下的宝兴百合进行转录组学分析，以探讨参与宝兴百合耐寒性的候选基因和途径。与对照处理相比，我们发现了 9600 个上调的差异表达基因（DEGs）和 7055 个下调的 DEGs。大量的 DEGs 参与了钙信号通路、丝裂原活化蛋白激酶（MAPK）级联、植物激素信号通路和脂代谢。共发现 400 个转录因子对低温胁迫有响应，其中大多数属于 MYB 和 AP2/ERF 家族。发现了五个新的基因可能是参与宝兴百合耐寒性的候选基因。该研究为进一步研究宝兴百合在寒冷胁迫下的生存机制提供了线索。
