Indole Diterpene Derivatives from the GZWMJZ-288, an Endophytic Fungus from .

A new indole diterpene, 26-dihydroxyaflavininyl acetate (), along with five known analogs (-) were isolated from the liquid fermentation of GZWMJZ-288, an endophyte from . The structures of these compounds were identified through NMR, MS, chemical reaction, and X-ray diffraction experiments. Enzyme inhibition activity screening found that compounds , , and have a good binding affinity with NPC1L1, among which compound exhibited a stronger binding ability than ezetimibe at a concentration of 10 µM. Moreover, compound showed inhibitory activity against -glucosidase with an IC value of 29.22 ± 0.83 µM, which is 13 times stronger than that of acarbose. The results suggest that these aflavinine analogs may serve as lead compounds for the development of drugs targeting NPC1L1 and -glucosidase. The binding modes of the bioactive compounds with NPC1L1 and α-glucosidase were also performed through in silico docking studies.