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[具体基因名称]的转录调控级联反应影响[具体植物名称]中的低温和干旱胁迫响应。

Transcriptional regulatory cascade of and affects low-temperature and drought stress response in .

作者信息

Zang Dandan, Sun Yan, Zhao Hengtian

机构信息

Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China.

出版信息

Front Plant Sci. 2023 Nov 27;14:1288947. doi: 10.3389/fpls.2023.1288947. eCollection 2023.

DOI:10.3389/fpls.2023.1288947
PMID:38089802
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10711284/
Abstract

The development of stress tolerance is regulated via the transcriptional regulatory networks involving regulatory homeostasis mediated by protein-DNA interactions. from was characterized to understand the underlying mechanism of low-temperature and drought stress response in . To better understand the transcription pathway of , we cloned the promoter and screened proteins that could interact with the promoter. Using Yeast one-hybrid, electrophoretic mobility shift, and chromatin immunoprecipitation assays, we found that the LcMYB71 protein specifically bound to the promoter of . The transient transformation and stable transgenic system were used to produce transgenic plants with overexpressed and silenced , elucidating the effect of on low-temperature and drought stress tolerance. positively regulated the proline content and enhanced the scavenging of reactive oxygen species, thus improving tolerance to low-temperature and drought stress. Further studies revealed that and had similar functions and could improve plant low-temperature and drought tolerance. It is necessary to identify the upstream regulators of a specific gene to characterize gene functions and the associated transcriptional pathways.

摘要

胁迫耐受性的发展是通过涉及由蛋白质 - DNA 相互作用介导的调节稳态的转录调控网络来调节的。为了了解[具体植物名称]中低温和干旱胁迫响应的潜在机制，对[相关植物]进行了表征。为了更好地理解[相关基因]的转录途径，我们克隆了启动子并筛选了可与该启动子相互作用的蛋白质。通过酵母单杂交、电泳迁移率变动分析和染色质免疫沉淀分析，我们发现 LcMYB71 蛋白特异性结合[相关基因]的启动子。利用瞬时转化和稳定转基因系统产生[相关植物]过表达和沉默[相关基因]的转基因植物，阐明了[相关基因]对低温和干旱胁迫耐受性的影响。[相关基因]正向调节脯氨酸含量并增强活性氧的清除能力，从而提高对低温和干旱胁迫的耐受性。进一步研究表明，[相关基因 1]和[相关基因 2]具有相似功能，均可提高植物的低温和干旱耐受性。有必要鉴定特定基因的上游调节因子，以表征基因功能和相关的转录途径。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/68c1ed8fa33b/fpls-14-1288947-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/201482151670/fpls-14-1288947-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/ad19a4ed7041/fpls-14-1288947-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/a2fefc31502a/fpls-14-1288947-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/883b14a1264c/fpls-14-1288947-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/15d80d5c4cdd/fpls-14-1288947-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/ef91f57ba766/fpls-14-1288947-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/08004807a7b0/fpls-14-1288947-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/551f083f837a/fpls-14-1288947-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/68c1ed8fa33b/fpls-14-1288947-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/201482151670/fpls-14-1288947-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/ad19a4ed7041/fpls-14-1288947-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/a2fefc31502a/fpls-14-1288947-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/883b14a1264c/fpls-14-1288947-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/15d80d5c4cdd/fpls-14-1288947-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/ef91f57ba766/fpls-14-1288947-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/08004807a7b0/fpls-14-1288947-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/551f083f837a/fpls-14-1288947-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6363/10711284/68c1ed8fa33b/fpls-14-1288947-g009.jpg

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