Li Yuting, Zhang Xiaoxi, Ye Jiabao, Xu Feng, Zhang Weiwei, Liao Yongling, Yang Xiaoyan
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China.
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, Hubei, China.
Plant Sci. 2024 Feb;339:111948. doi: 10.1016/j.plantsci.2023.111948. Epub 2023 Dec 12.
Although long non-coding RNAs have been recognized to play important roles in plant, their possible functions and potential mechanism in Ginkgo biloba flavonoid biosynthesis are poorly understood. Flavonoids are important secondary metabolites and healthy components of Ginkgo biloba. They have been widely used in food, medicine, and natural health products. Most previous studies have focused on the molecular mechanisms of structural genes and transcription factors that regulate flavonoid biosynthesis. Few reports have examined the biological functions of flavonoid biosynthesis by long non-coding RNAs in G. biloba. Long noncoding RNAs associated with flavonoid biosynthesis in G. biloba have been identified through RNA sequencing, but the function of lncRNAs has not been reported. In this study, the expression levels of lnc10 and lnc11 were identified. Quantitative real-time polymerase chain reaction analysis revealed that lnc10 and lnc11 were expressed in all detected organs, and they showed significantly higher levels in immature and mature leaves than in other organs. In addition, to fully identify the function of lnc10 and lnc11 in flavonoid biosynthesis in G. biloba, lnc10 and lnc11 were cloned from G. biloba, and were transformed into Arabidopsis and overexpressed. Compared with the wild type, the flavonoid content was increased in transgenic plants. Moreover, the RNA-sequencing analysis of wild-type, lnc10-overexpression, and lnc11-overexpression plants screened out 2019 and 2552 differentially expressed genes, and the transcript levels of structural genes and transcription factors associated with flavonoid biosynthesis were higher in transgenic Arabidopsis than in the wild type, indicating that lnc10 and lnc11 activated flavonoid biosynthesis in the transgenic lines. Overall, these results suggest that lnc10 and lnc11 positively regulate flavonoid biosynthesis in G. biloba.
尽管长链非编码RNA已被认为在植物中发挥重要作用，但其在银杏黄酮生物合成中的可能功能和潜在机制仍知之甚少。黄酮类化合物是银杏重要的次生代谢产物和健康成分。它们已广泛应用于食品、医药和天然保健品中。以往大多数研究都集中在调控黄酮生物合成的结构基因和转录因子的分子机制上。很少有报道研究长链非编码RNA在银杏黄酮生物合成中的生物学功能。通过RNA测序已鉴定出与银杏黄酮生物合成相关的长链非编码RNA，但lncRNA的功能尚未见报道。在本研究中，鉴定了lnc10和lnc11的表达水平。定量实时聚合酶链反应分析表明，lnc10和lnc11在所有检测的器官中均有表达，且在未成熟叶和成熟叶中的表达水平显著高于其他器官。此外，为了全面鉴定lnc10和lnc11在银杏黄酮生物合成中的功能，从银杏中克隆了lnc10和lnc11，并将其转化到拟南芥中进行过表达。与野生型相比，转基因植物中的黄酮含量增加。此外，对野生型、lnc10过表达和lnc11过表达植物的RNA测序分析筛选出了2019个和2552个差异表达基因，与黄酮生物合成相关的结构基因和转录因子的转录水平在转基因拟南芥中高于野生型，表明lnc10和lnc11激活了转基因株系中的黄酮生物合成。总体而言，这些结果表明lnc10和lnc11正向调控银杏黄酮的生物合成。
