Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, School of Food and Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
Food Chem. 2024 May 15;440:138184. doi: 10.1016/j.foodchem.2023.138184. Epub 2023 Dec 14.
Rapid assessment of microbiological quality (i.e., Total Aerobic Counts, TAC) and authentication (i.e., fresh vs frozen/thawed) of meat was investigated using spectroscopic-based methods. Data were collected throughout storage experiments from different conditions. In total 526 spectra (Fourier transform infrared, FTIR) and 534 multispectral images (MSI) were acquired. Partial Least Squares (PLS) was applied to select/transform the variables. In the case of FTIR data 30 % of the initial features were used, while for MSI-based models all features were employed. Subsequently, Support Vector Machines (SVM) regression/classification models were developed and evaluated. The performance of the models was evaluated based on the external validation set. In both cases MSI-based models (Root Mean Square Error, RMSE: 0.48-1.08, Accuracy: 91-97 %) were slightly better compared to FTIR (RMSE: 0.83-1.31, Accuracy: 88-94 %). The most informative features of FTIR for the case of quality were mainly in 900-1700 cm, while for fraud the features were more dispersed.
使用基于光谱的方法研究了快速评估肉的微生物质量(即总需氧菌计数,TAC)和真伪(即新鲜与冷冻/解冻)。在不同条件下的储存实验中收集了数据。共采集了 526 个光谱(傅里叶变换红外光谱,FTIR)和 534 个多光谱图像(MSI)。偏最小二乘法(PLS)用于选择/转换变量。在 FTIR 数据的情况下,使用了初始特征的 30%,而对于基于 MSI 的模型,则使用了所有特征。随后,开发并评估了支持向量机(SVM)回归/分类模型。根据外部验证集评估了模型的性能。在这两种情况下,基于 MSI 的模型(均方根误差,RMSE:0.48-1.08,准确性:91-97%)略优于 FTIR(RMSE:0.83-1.31,准确性:88-94%)。对于质量案例,FTIR 最具信息量的特征主要在 900-1700cm 之间,而对于欺诈,特征则更为分散。