育龄期女性阴道微生物群与子宫内膜息肉样病变的相关性：一项横断面研究。

Associations between vaginal microbiota and endometrial polypoid lesions in women of reproductive age: a cross-sectional study.

作者信息

Tian Zhaomei, Zhao Maoning, Sui Xinlei, Li Xiao, Qin Lang, Chen Zi-Jiang, Zhao Shigang, Zhao Han

机构信息

State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong, China; National Research Centre for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China.

State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong, China; Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Jinan, Shandong, China; National Research Centre for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China; Research Unit of Gametogenesis and Health of ART-Offspring, Chinese Academy of Medical Sciences, Jinan, Shandong, China; Shandong Key Laboratory of Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China; Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, China; Centre for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

出版信息

Reprod Biomed Online. 2024 Feb;48(2):103602. doi: 10.1016/j.rbmo.2023.103602. Epub 2023 Oct 11.

DOI:10.1016/j.rbmo.2023.103602

PMID:38101145

Abstract

RESEARCH QUESTION

What are the different characteristics of vaginal microbial composition between patients with endometrial polypoid lesions and controls?

DESIGN

This cohort study compared the pre-operative microbial compositions of vaginal samples in a cohort of 703 women with endometrial polypoid lesions [293 and 410 women diagnosed and not diagnosed with polyps pathologically (polyps group and not-polyps group, respectively] and 703 women in the control group. Bacterial abundance, diversity, differential taxa and microbial network structure were assessed using 16S rRNA gene sequencing. Predictive algorithms were used to determine the functional pathways of vaginal microbiota within the cohort.

RESULTS

The control group exhibited higher relative abundance of Lactobacillus crispatus in comparison with the polypoid lesions group (P = 0.0427). Beta diversity of vaginal microbiota differed significantly between the groups (P < 0.05). Comparing the polyps group with the not-polyps group, Leptotrichia spp. and Cutibacterium spp. were more abundant in the polyps group, and Fannyhessea spp., Acinetobacter spp. and Achromobacter spp. were more abundant in the not-polyps group. The control group exhibited higher abundance of Bifidobacterium spp., Achromobacter spp. and Escherichia/Shigella spp. (false discovery rate < 0.05). Furthermore, the polyps group and not-polyps group displayed more complex co-occurrence networks compared with the control group.

CONCLUSIONS

The results of this study provide compelling evidence supporting associations between vaginal microbiota and endometrial polypoid lesions, highlighting the potential relationship between a well-balanced vaginal microbial ecosystem and a healthy intrauterine environment.

摘要

研究问题

子宫内膜息肉样病变患者与对照组之间阴道微生物组成的不同特征是什么？

设计

这项队列研究比较了703例患有子宫内膜息肉样病变的女性（其中293例和410例分别经病理诊断为有息肉和无息肉，分别为息肉组和非息肉组）以及703例对照组女性术前阴道样本的微生物组成。使用16S rRNA基因测序评估细菌丰度、多样性、差异分类群和微生物网络结构。采用预测算法确定队列中阴道微生物群的功能途径。

结果

与息肉样病变组相比，对照组卷曲乳杆菌的相对丰度更高（P = 0.0427）。两组之间阴道微生物群的β多样性存在显著差异（P < 0.05）。息肉组与非息肉组相比，纤毛菌属和棒状杆菌属在息肉组中更为丰富，而范海斯菌属、不动杆菌属和无色杆菌属在非息肉组中更为丰富。对照组双歧杆菌属、无色杆菌属和埃希氏菌属/志贺氏菌属的丰度更高（错误发现率 < 0.05）。此外，与对照组相比，息肉组和非息肉组显示出更复杂的共现网络。