TRAPs：“带操作员的电梯”机制。

TRAPs: the 'elevator-with-an-operator' mechanism.

机构信息

Department of Biochemistry and Biophysics, Stockholm University, 106 91 Stockholm, Sweden.

Biomolecular Interaction Centre, Maurice Wilkins Centre for Biodiscovery, MacDiarmid Institute for Advanced Materials and Nanotechnology, Christchurch 8140, New Zealand; School of Biological Sciences, University of Canterbury, Christchurch 8140, New Zealand.

出版信息

Trends Biochem Sci. 2024 Feb;49(2):134-144. doi: 10.1016/j.tibs.2023.11.006. Epub 2023 Dec 14.

DOI:10.1016/j.tibs.2023.11.006

PMID:38102017

Abstract

Tripartite ATP-independent periplasmic (TRAP) transporters are nutrient-uptake systems found in bacteria and archaea. These evolutionary divergent transporter systems couple a substrate-binding protein (SBP) to an elevator-type secondary transporter, which is a first-of-its-kind mechanism of transport. Here, we highlight breakthrough TRAP transporter structures and recent functional data that probe the mechanism of transport. Furthermore, we discuss recent structural and biophysical studies of the ion transporter superfamily (ITS) members and highlight mechanistic principles that are relevant for further exploration of the TRAP transporter system.

摘要

三磷酸非依赖的周质（TRAP）转运蛋白是在细菌和古菌中发现的营养物质摄取系统。这些进化上有差异的转运蛋白系统将底物结合蛋白（SBP）与提升式二级转运蛋白偶联在一起，这是一种首创的运输机制。在这里，我们重点介绍突破性的 TRAP 转运蛋白结构和最近的功能数据，这些数据探讨了运输的机制。此外，我们还讨论了离子转运蛋白超家族（ITS）成员的最新结构和生物物理研究，并强调了与进一步探索 TRAP 转运蛋白系统相关的机制原则。