State Environmental Protection Key Laboratory of Food Chain Pollution Control, School of Ecology and Environment, Beijing Technology and Business University, Beijing, 100048, China.
Key Laboratory of Changjiang Regulation and Protection of Ministry of Water Resources, Changjiang Institude of Survey Planning Design and Research, Wuhan, 430010, China.
Environ Sci Pollut Res Int. 2024 Jan;31(3):4400-4411. doi: 10.1007/s11356-023-31461-0. Epub 2023 Dec 16.
Biological soil crusts (BSCs) are common in arid and semi-arid ecosystems and enhance soil stability and fertility. Highway slopes severely deplete the soil ecological structure and soil nutrients, hindering plant survival. The construction of highway slope BSCs under human intervention is critical to ensure the long-term stable operation of the slope ecosystem. This study investigated the variation rules and interaction mechanisms between soil nutrients and microbial communities in the subsoil BSCs on highway slopes. Bacterial 16S rRNA high-throughput sequencing was employed to investigate the dynamic compositional changes in the microbial community and perform critical metabolic predictive analyses of functional bacteria. This study revealed that the total soil nitrogen increased significantly from 0.557 to 0.864 g/kg after artificial inoculation with desert Phormidium tenue and Scytonema javanicum. Actinobacteria (44-48%) and Proteobacteria (28-31%) were the dominant phyla in all samples. The abundance of Cyanobacteria, Cytophagaceae, and Chitinophagaceae increased significantly after inoculation. PICRUST analysis showed that the main metabolic pathways of soil microorganisms on highway slopes included cofactor and vitamin, nucleotide, and amino acid metabolisms. These findings suggest that the artificial inoculation with Phormidium tenue and Scytonema javanicum could alter soil microbial distribution to promote soil development on highway slopes toward nutrient accumulation.
生物土壤结皮（BSCs）在干旱和半干旱生态系统中很常见，它可以增强土壤稳定性和肥力。高速公路边坡严重破坏土壤生态结构和土壤养分，阻碍植物生存。在人为干预下，高速公路边坡 BSCs 的建设对于确保边坡生态系统的长期稳定运行至关重要。本研究调查了高速公路边坡下土壤养分和微生物群落之间的变化规律和相互作用机制。采用细菌 16S rRNA 高通量测序技术，研究了微生物群落的动态组成变化，并对功能细菌进行了关键代谢预测分析。研究表明，人工接种荒漠发网藻和拟束丝藻后，土壤全氮从 0.557 显著增加到 0.864 g/kg。在所有样本中，放线菌（44-48%）和变形菌（28-31%）是主要的门。接种后蓝藻、噬纤维菌科和几丁质噬纤维菌科的丰度显著增加。PICRUST 分析表明，高速公路边坡土壤微生物的主要代谢途径包括辅因子和维生素、核苷酸和氨基酸代谢。这些发现表明，人工接种荒漠发网藻和拟束丝藻可以改变土壤微生物的分布，促进高速公路边坡土壤的发育，实现养分积累。
