Zhou Xiangjun, Liang Bin, Zhang Tian, Xiong Qiao, Ma Xiao, Chen Lanzhou
Huangshi Key Laboratory of Prevention and Control of Soil Pollution, College of Urban and Environmental Sciences, Hubei Normal University, Huangshi, China.
Hubei Key Laboratory of Biomass-Resources Chemistry and Environmental Biotechnology, School of Resource and Environmental Sciences, Wuhan University, Wuhan, China.
Front Microbiol. 2024 Apr 8;15:1377732. doi: 10.3389/fmicb.2024.1377732. eCollection 2024.
The inoculation of cyanobacteria for enriching soil nutrients and forming biological soil crusts (BSCs) is considered an effective means to restore degraded soil. However, there are limited studies on the application of co-inoculation of fungi and cyanobacteria for degraded soil remediation. In this study, a high exopolysaccharide-secreting fungi Zh2 was isolated from lichen BSCs in Hobq Desert, and co-inoculated with a cyanobacterial strain identified as in different proportions to form BSCs on sand during a 35 days incubation period. Results revealed significant differences in crust biomass and soil properties among crusts with different cyanobacterial/fungal inoculation ratios. Microbial biomass, soil nutrient content and enzyme activities in crusts co-inoculated with cyanobacteria and fungi were higher than those inoculated with cyanobacteria and fungi alone. The inoculation of cyanobacteria contributed to the fulvic-like accumulation, and the inoculated fungi significantly increased the humic-like content and soil humification. Redundancy analysis showed that the inoculation of cyanobacteria was positively correlated with the activities of urease and phosphatase, and the content of fulvic-like. Meanwhile, the inoculation of fungi was positively correlated with the contents of total carbon, total nitrogen and humic-like, the activities of catalase and sucrase. Cyanobacteria and fungi play distinct roles in improving soil fertility and accumulating dissolved organic matter. This study provides new insights into the effects of cyanobacteria and fungi inoculations on the formation and development of cyanobacterial-fungus complex crusts, offering a novel method for accelerating induced crust formation on the surface of sand.
接种蓝细菌以富集土壤养分并形成生物土壤结皮（BSCs）被认为是恢复退化土壤的有效手段。然而，关于真菌和蓝细菌共同接种用于退化土壤修复的应用研究有限。在本研究中，从毛乌素沙漠的地衣生物土壤结皮中分离出一种高分泌胞外多糖的真菌Zh2，并在35天的培养期内与一种鉴定为 的蓝细菌菌株以不同比例共同接种，在沙子上形成生物土壤结皮。结果显示，不同蓝细菌/真菌接种比例的结皮在结皮生物量和土壤性质方面存在显著差异。蓝细菌和真菌共同接种的结皮中的微生物生物量、土壤养分含量和酶活性高于单独接种蓝细菌和真菌的结皮。接种蓝细菌有助于类富里酸的积累，接种真菌显著增加了类胡敏酸含量和土壤腐殖化程度。冗余分析表明，接种蓝细菌与脲酶和磷酸酶活性以及类富里酸含量呈正相关。同时，接种真菌与总碳、总氮和类胡敏酸含量、过氧化氢酶和蔗糖酶活性呈正相关。蓝细菌和真菌在提高土壤肥力和积累溶解有机物方面发挥着不同的作用。本研究为蓝细菌和真菌接种对蓝细菌-真菌复合结皮形成和发育的影响提供了新的见解，为加速沙地表面诱导结皮的形成提供了一种新方法。
