University Clinic for Swine, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria.
Institute of Immunology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria.
Res Vet Sci. 2024 Jan;166:105109. doi: 10.1016/j.rvsc.2023.105109. Epub 2023 Dec 13.
Chlamydia (C.) suis, a zoonotic intracellular bacterium, is described as a causative agent for conjunctivitis, particularly in nursery and fattening pigs. Chlamydiaceae are claimed to survive drying and to persist in dust. The objective of this study was to evaluate the viability of Chlamydia in dust sampled in a fattening pig farm with a high appearance of chlamydial-induced conjunctivitis. Dust was collected and stored at room temperature. To evaluate bacterial load and survival over time, quantitative PCR (Chlamydiaceae, C. suis) and isolation in cell culture were performed every week for up to 16 weeks. While qPCR results remained highly positive with consistent bacterial loads between 10 and 10 copy numbers/100 μL eluate over a period of 16 weeks and even after 40 weeks, it was not possible to isolate Chlamydia except for the initial sample. These results show only short-term viability of C. suis in dust. This is an important information regarding reduction of chlamydial loads in pig farms and risk for pigs and people to get infected via dust.
猪衣原体（C. suis）是一种动物源细胞内细菌，被描述为引起结膜炎的病原体，特别是在育肥猪和仔猪中。据称，衣原体科在干燥条件下能够存活，并在尘埃中持续存在。本研究的目的是评估在高发性衣原体诱导性结膜炎育肥猪场内采集的尘埃中衣原体的生存能力。采集尘埃并在室温下储存。为了评估随时间推移的细菌负荷和生存能力，每周进行定量 PCR（衣原体科、猪衣原体）和细胞培养分离，最长可达 16 周。虽然 qPCR 结果在 16 周内一直呈高度阳性，细菌负荷保持在 10 到 10 拷贝数/100 μL 洗脱液之间，甚至在 40 周后也是如此，但除了初始样本外，无法分离出衣原体。这些结果表明，尘埃中猪衣原体的生存能力仅为短期。这对于减少猪场内的衣原体负荷以及猪和人通过尘埃感染的风险具有重要意义。
