Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China; Gansu Gaolan Field Scientific Observation and Research Station for Agricultural Ecosystem, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Gansu Shangnong Biotechnology Co. Ltd, Baiyin 730900, China.
Sci Total Environ. 2024 Feb 20;912:169371. doi: 10.1016/j.scitotenv.2023.169371. Epub 2023 Dec 16.
The constraint of phosphorus (P) fixation on crop production in alkaline calcareous soils can be alleviated by applying bioinoculants. However, the impact of bacterial inoculants on this process remains inadequately understood. Here, a field study was conducted to investigate the effect of a high-concentration, cost-effective, and slow-release granular bacterial inoculant (GBI) on maize (Zea mays L.) plant growth. Additionally, we explored the effects of GBI on rhizosphere soil aggregate physicochemical properties, rhizosphere soil P fraction, and microbial communities within aggregates. The outcomes showed a considerable improvement in plant growth and P uptake upon application of the GBI. The application of GBI significantly enhanced the AP, phoD gene abundance, alkaline phosphatase activity, inorganic P fractions, and organic P fractions in large macroaggregates. Furthermore, GBI impacted soil aggregate fractionation, leading to substantial alterations in the composition of fungal and bacterial communities. Notably, key microbial taxa involved in P-cycling, such as Saccharimonadales and Mortierella, exhibited enrichment in the rhizosphere soil of plants treated with GBI. Overall, our study provides valuable insight into the impact of GBI application on microbial distributions and P fractions within aggregates of alkaline calcareous soils, crucial for fostering healthy root development and optimal crop growth potential. Subsequent research endeavors should delve into exploring the effects of diverse GBIs and specific aggregate types on P fraction and community composition across various soil profiles.
在碱性石灰性土壤中，磷（P）固定会限制作物生产，可以通过施加生物接种剂来缓解。然而，细菌接种剂对这一过程的影响仍未得到充分理解。在这里，进行了一项田间研究，以调查高浓度、具有成本效益且缓释颗粒状细菌接种剂（GBI）对玉米（Zea mays L.）植物生长的影响。此外，我们还探讨了 GBI 对根际土壤团聚体物理化学性质、根际土壤 P 形态和团聚体内微生物群落的影响。结果表明，施用 GBI 可显著促进植物生长和 P 吸收。应用 GBI 可显著提高碱性磷酸酶活性、AP、phoD 基因丰度、无机 P 形态和大宏团聚体中的有机 P 形态。此外，GBI 影响土壤团聚体分形，导致真菌和细菌群落组成发生重大变化。值得注意的是，与 P 循环相关的关键微生物类群，如 Saccharimonadales 和 Mortierella，在 GBI 处理的植物根际土壤中丰度增加。总的来说，我们的研究深入了解了 GBI 应用对碱性石灰性土壤团聚体中微生物分布和 P 形态的影响，这对于促进健康的根系发育和最佳作物生长潜力至关重要。后续的研究工作应深入探讨不同 GBI 和特定团聚体类型对不同土壤剖面中 P 形态和群落组成的影响。
