电针通过抑制线粒体信号通路减轻晶状体诱导的近视豚鼠睫状肌细胞凋亡。
Electroacupuncture alleviates ciliary muscle cell apoptosis in lens-induced myopic guinea pigs through inhibiting the mitochondrial signaling pathway.
作者信息
Yang Zhao-Hui, Hao Jia-Wen, Liu Jin-Peng, Bao Bo, Li Tu-Ling, Wu Qiu-Xin, He Ming-Guang, Bi Hong-Sheng, Guo Da-Dong
机构信息
Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China.
Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Shandong Provincial Clinical Medical Research Center of Optometry and Adolescent Low Vision Prevention and Control, Jinan 250002, Shandong Province, China.
出版信息
Int J Ophthalmol. 2023 Dec 18;16(12):1942-1951. doi: 10.18240/ijo.2023.12.05. eCollection 2023.
AIM
To investigate the effect of electroacupuncture (EA) on the mitochondria-dependent apoptotic signaling pathway in the ciliary muscle of guinea pigs with negative lens-induced myopia (LIM).
METHODS
Guinea pigs were randomly divided into normal control (NC) group, LIM group, LIM+SHAM acupoint (LIM+SHAM) group, and LIM+EA group. Animals in the NC group received no intervention, while those in other three groups were covered with -6.0 diopter (D) lenses on right eyes. Meanwhile, animals in the LIM+EA group received EA at Hegu (LI4) combined with Taiyang (EX-HN5) acupoints, while those in the LIM+SHAM group were treated at sham points. After treatments for 1, 2, and 4wk, morphological changes in ciliary muscles were observed with hematoxylin and eosin (H&E) staining and nick end labeling (TUNEL), and the expression of the mitochondrial apoptotic signaling pathway-related molecules in ciliary muscles was measured by real-time quantitative polymerase chain reaction (qPCR) and Western blot. Additionally, the adenosine triphosphate (ATP) contents were also determined in ciliary muscles.
RESULTS
Axial length increased significantly in the LIM and LIM+SHAM groups and decreased in the LIM+EA group. The ciliary muscle fibers were broken and destroyed in both LIM and LIM+SHAM groups, whereas those in the LIM+EA group improved significantly. TUNEL assay showed the number of apoptotic cells increased in the LIM and LIM+SHAM groups, whereas reduced in the LIM+EA group. ATP contents showed a significant decrease in the LIM and LIM+SHAM groups, whereas increased after EA treatment. Compared with the NC group, the dynamin-related protein 1 (DRP1), Caspase3, and apoptotic protease activator 1 (APAF1) levels were significantly increased in the LIM group and decreased in the LIM+EA group.
CONCLUSION
The results provide evidence of EA inhibiting the development of myopia by regulating the mitochondrial apoptotic signaling pathway.
目的
探讨电针（EA）对负透镜诱导型近视（LIM）豚鼠睫状肌线粒体依赖性凋亡信号通路的影响。
方法
将豚鼠随机分为正常对照组（NC组）、LIM组、LIM+假穴组（LIM+SHAM组）和LIM+EA组。NC组动物不接受干预，其他三组动物右眼佩戴-6.0屈光度（D）的透镜。同时，LIM+EA组动物在合谷（LI4）穴与太阳穴（EX-HN5）进行电针治疗，而LIM+SHAM组动物在假穴进行治疗。治疗1、2和4周后，用苏木精-伊红（H&E）染色和缺口末端标记法（TUNEL）观察睫状肌的形态变化，并用实时定量聚合酶链反应（qPCR）和蛋白质免疫印迹法检测睫状肌中线粒体凋亡信号通路相关分子的表达。此外，还测定了睫状肌中的三磷酸腺苷（ATP）含量。
结果
LIM组和LIM+SHAM组的眼轴长度显著增加，而LIM+EA组的眼轴长度减少。LIM组和LIM+SHAM组的睫状肌纤维均断裂和破坏，而LIM+EA组的睫状肌纤维明显改善。TUNEL检测显示，LIM组和LIM+SHAM组的凋亡细胞数量增加，而LIM+EA组的凋亡细胞数量减少。ATP含量在LIM组和LIM+SHAM组显著降低，而电针治疗后升高。与NC组相比，LIM组的动力相关蛋白1（DRP1）、半胱天冬酶3（Caspase3）和凋亡蛋白酶激活因子1（APAF1）水平显著升高，而LIM+EA组降低。
结论
结果表明电针可通过调节线粒体凋亡信号通路抑制近视的发展。
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